Magnetically Actuated Biohybrid Microswimmers for Precise Photothermal Muscle Contraction
Magnetically Actuated Biohybrid Microswimmers for Precise Photothermal Muscle Contraction
复制标题
用于精确光热肌肉收缩的磁驱动生物混合微型游泳器
DOI：
10.1021/acsnano.2c00833
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发表时间：
2022-03-15
期刊：
影响因子：
17.1
通讯作者：
Tu, Yingfeng
中科院分区：
文献类型：
--
作者：
Liu, Lu;Wu, Juanyan;Tu, Yingfeng
近年来，针对骨骼肌组织工程和仿生学等领域，设计了多种肌管收缩和骨骼肌刺激策略。然而，目前大多数方法缺乏对精确刺激的可控性和适应性，特别是在微观层面。本文成功地演示了利用磁性生物混合微游泳器结合近红外激光照射无线和精确激活肌肉。将超顺磁性的Fe3O4纳米颗粒浸渍在小球藻微藻上，制备出生物杂交微游泳体，使其在磁性驱动下在各种生物介质中具有强大的导航能力。在旋转磁场的引导下，设计的微游泳者可以实现对单个c2c12衍生肌管的精确运动。在近红外照射下，掺入的Fe3O4纳米颗粒的光热效应导致靶肌管局部温度增加约5℃，从而有效地触发肌管收缩。这种现象背后的机制是Ca2+独立的情况下，涉及直接肌动蛋白-肌球蛋白相互作用。体内肌纤维收缩和组织学试验进一步证明了我们设计的有效性和生物安全性。基于微游泳者的生物混合策略可能为组织工程和仿生学提供一种革新。
Various strategies have been designed for myotube contraction and skeletal muscle stimulation in recent years, aiming in the field of skeletal muscle tissue engineering and bionics. However, most of the current approaches lack controllability and adaptability for precise stimulation, especially at the microlevel. Herein, wireless and precise activation of muscle by using magnetic biohybrid microswimmers in combination with near-infrared (NIR) laser irradiation is successfully demonstrated. Biohybrid microswimmers are fabricated by dip-coating superparamagnetic Fe3O4 nanoparticles onto the chlorella microalgae, thus endowing robust navigation in various biological media due to magnetic actuation. Under the guidance of a rotating magnetic field, the engineered microswimmer can achieve precise motion toward a single C2C12-derived myotube. Upon NIR irradiation, the photothermal effect from the incorporated Fe3O4 nanoparticles results in local temperature increments of approximately 5 degrees C in the targeted myotube, which could efficiently trigger the contraction of myotube. The mechanism underlying this phenomenon is a Ca2+-independent case involving direct actin-myosin interactions. In vivo muscle fiber contraction and histological test further demonstrate the effectiveness and biosafety of our design. The as-developed biohybrid microswimmer-based strategy is possible to provide a renovation for tissue engineering and bionics.