Overexpression of transfected cathepsin D in transformed cells increases their malignant phenotype and metastatic potency.
Overexpression of transfected cathepsin D in transformed cells increases their malignant phenotype and metastatic potency.
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发表时间：
1990-12
期刊：
影响因子：
8
通讯作者：
Marcel Garcia;D. Derocq;Pascal Pujol;Henri Rochefort
中科院分区：
文献类型：
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作者：
Marcel Garcia;D. Derocq;Pascal Pujol;Henri Rochefort
组织蛋白酶D是一种天冬氨酸氨基转移酶，在乳腺癌细胞中高表达，与肿瘤转移密切相关。我们用腺病毒转化的不分泌组织蛋白酶D的大鼠细胞株，研究了转染人组织蛋白酶D基因的后果。人组织蛋白酶D在稳定的转基因克隆中过表达，导致在低血清条件下较高的初始生长速度，在高细胞密度下过度生长导致星状聚集，以及在软琼脂中更大的锚定无关生长。裸鼠体内注射的组织蛋白酶D克隆的转移活性(主要在肝脏)显著高于对照克隆。这些结果表明，组织蛋白酶D的过表达增加了恶性细胞的体外转化表型和体内转移能力。
Cathepsin D, an aspartic lysosomal proteinase, is overexpressed by breast cancer cells and highly correlated with the occurrence of metastasis in patients. We used an adenovirus transformed rat cell line that does not secrete cathepsin D, to study the consequences of transfecting human cathepsin D cDNA. Overexpression of human cathepsin D in stable transfectant clones, results in higher initial growth rates in low serum conditions, overgrowth at high cell densities resulting in stellate aggregates, and greater anchorage-independent growth in soft agar. The metastatic activity (mostly in liver) of cathepsin D clones injected into athymic mice was significantly higher than that of control clones. These results show that overexpression of cathepsin D increases the transformed phenotype of malignant cells in vitro and their metastatic potency in vivo.