Molecular characterization of a signal-regulated kinase homolog from Echinococcus granulosus
Molecular characterization of a signal-regulated kinase homolog from Echinococcus granulosus
复制标题
细粒棘球绦虫信号调节激酶同源物的分子表征
DOI：
10.3760/cma.j.issn.0366-6999.2011.18.008
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发表时间：
2011-09-20
影响因子：
6.1
通讯作者：
Lin Ren-yong
中科院分区：
文献类型：
--
作者：
Li Jing;Zhang Chuan-shan;Lin Ren-yong
背景：细粒棘球蚴（E.细粒棘球蚴病（Granulosus）是最重要的慢性蠕虫病之一，尤其是在羊/牛饲养区。幼虫期的寄生虫形成一个包囊，生长在宿主的肝、肺或其他器官中。为了确保在宿主组织中的长寿命，寄生虫在其宿主之间建立复杂的细胞间通信系统，以允许其向每个幼虫阶段分化。最近的研究报道，这种通信与蠕虫寄生虫中的细胞外信号调节激酶（ERK）丝裂原活化蛋白激酶级联反应有关，特别是这些蛋白激酶可能作为一种新的囊型包虫病化疗的有效靶点。本研究的目的是研究一种新的ERK同源基因的生物学功能。方法从E.颗粒状体中，并使用LA Taq聚合酶链反应（PCR）方法和生物信息学进行分析。利用反转录PCR（RT-PCR）技术检测了该基因在两种不同组织中的转录水平。Western blotting检测细胞中EgERK蛋白的表达。结果从大肠杆菌中克隆了Egerk基因的全基因组，并在大肠杆菌中进行了表达谱分析。颗粒状。此外，还鉴定了Egerk的两种选择性剪接转录本Egerk-A和Egerk-B。发现Egerk-A在两种不同的幼虫组织（囊膜和原囊膜）中在转录和蛋白水平上组成型表达。Egerk-A在E.结论我们从大肠杆菌中克隆了一个新的ERK同源基因。颗粒状细胞，EgERK（GenBank ID HQ 585923），并发现其在囊膜和原头节中组成型表达。这些发现将有助于进一步研究该基因在棘球蚴生长发育中的生物学功能，并有助于研究新的抗棘球蚴病药物靶点。中华医学杂志2011;124（18）：2838-2844
Background Cystic echinococcosis due to Echinococcus granulosus (E. granulosus) is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. The larval stage of the parasite forms a cyst that grows in the liver, lung, or other organs ofthe host. To ensure a long life in the host tissues, the parasite establishes complex inter-cellular communication systems between its host to allow its differentiation toward each larval stage. Recent studies have reported that this communication is associated with the extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase cascade in helminth parasites, and in particular that these protein kinases might serve as effective targets for a novel chemotherapy for cystic echinococcosis. The aim of the present study investigated the biological function of a novel ERK ortholog from E. granulosus, EgERK.Methods DNA encoding EgERK was isolated from protoscolices of E. granulosus and analyzed using the LA Taq polymerase chain reaction (PCR) approach and bioinformatics. Reverse transcription PCR (RT-PCR) was used to determine the transcription level of the gene at two different larval tissues. Western blotting was used to detect levels of EgERK protein. The expression profile of EgERK in protoscolices was examined by immunofluorescence.Results We cloned the entire Egerk genomic locus from E. granulosus. In addition, two alternatively spliced transcripts of Egerk, Egerk-A, and Egerk-B were identified. Egerk-A was found to constitutively expressed at the transcriptional and protein levels in two different larval tissues (cyst membranes and protoscolices). Egerk-A was expressed in the tegumental structures, hooklets, and suckers and in the tissue surrounding the rostellum of E. granulosus protoscolices.Conclusions We have cloned the genomic DNA of a novel ERK ortholog from E. granulosus, EgERK (GenBank ID HQ585923), and found that it is constitutively expressed in cyst membrane and protoscolex. These findings will be useful in further study of the biological functions of the gene in the growth and development of Echinococcus and will contribute to research on novel anti-echinococcosis drug targets. Chin Med J 2011;124(18):2838-2844