R-α lipoic acid γ-cyclodextrin complex increases energy expenditure: A 4-month feeding study in mice
R-α lipoic acid γ-cyclodextrin complex increases energy expenditure: A 4-month feeding study in mice
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DOI：
10.1016/j.nut.2013.08.002
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发表时间：
2014-02-01
期刊：
影响因子：
4.4
通讯作者：
Rimbach, Gerald
中科院分区：
文献类型：
--
作者：
Nikolai, Sibylle;Huebbe, Patricia;Rimbach, Gerald
目的：高脂饮食（HFD）对实验鼠能量消耗的影响。R-α-硫辛酸环糊精（RALA-CD）复合物是硫辛酸（LA）的稳定形式，并且可以改善能量消耗。本研究的目的是确定RALA-CD对雌性实验室小鼠能量消耗和潜在分子靶点的影响。方法：雌性C57 BL/6 J小鼠饲喂含0.1%LA的HFD约16周。能量消耗，基因和蛋白质表达的影响进行了评估，采用间接量热法，实时逆转录聚合酶链反应，和Western blot，respectively.Results：补充小鼠RALA-CD导致能量消耗显着增加。然而，RALA本身（不含γ-环糊精）和S-cc硫辛酸环糊精都没有显著改变能量消耗。此外，RALA-CD改变了编码能量代谢相关蛋白的基因表达。转录关键调节因子sirtuin 3和过氧化物酶体增殖物激活受体-γ，共激活因子1 α，以及甲状腺相关酶2型碘甲状腺原氨酸脱碘酶在RALA-CD喂养小鼠的棕色脂肪组织（BAT）中上调。重要的是，下游效应解偶联蛋白（UCP）1和3的mRNA和/或蛋白质表达也升高BAT从RALA-CD补充mice.Conclusion：总体而言，目前的数据表明，RALA-CD是一个调节器的能量消耗在实验室小鼠。(C)2014爱思唯尔公司All rights reserved.
Objective: A high-fat diet (HFD) affects energy expenditure in laboratory rodents. R-alpha lipoic acid cyclodextrin (RALA-CD) complex is a stable form of lipoic acid (LA) and may improve energy expenditure. The aim of this study was to determine the effect of RALA-CD on energy expenditure and underlying molecular targets in female laboratory mice.Methods: Female C57BL/6J mice were fed a HFD containing 0.1% LA for about 16 wk. The effects on energy expenditure, gene and protein expression were assessed using indirect calorimetry, real-time reverse transcriptase polymerase chain reaction, and Western blot, respectively.Results: Supplementing mice with RALA-CD resulted in a significant increase in energy expenditure. However, both RALA per se (without gamma-cyclodextrin) and S-cc lipoic acid cyclodextrin did not significantly alter energy expenditure. Furthermore RALA-CD changed expression of genes encoding proteins centrally involved in energy metabolism. Transcriptional key regulators sirtuin 3 and peroxisome proliferator-activated receptor-gamma, coactivator 1 alpha, as well as thyroid related enzyme type 2 iodothyronine deiodinase were up-regulated in brown adipose tissue (BAT) of RALA-CD-fed mice. Importantly, mRNA and/or protein expression of downstream effectors uncoupling protein (Ucp) 1 and 3 also were elevated in BAT from RALA-CD-supplemented mice.Conclusion: Overall, present data suggest that RALA-CD is a regulator of energy expenditure in laboratory mice. (C) 2014 Elsevier Inc. All rights reserved.