Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells
Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells
复制标题
鳖肽调节人胃癌 AGS 细胞中的 microRNA 谱
DOI：
10.3892/ol.2017.7692
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发表时间：
2018-03-01
期刊：
影响因子：
2.9
通讯作者：
Ling, Zhi-Qiang
中科院分区：
文献类型：
--
作者：
Wu, Yi-Chen;Liu, Xiang;Ling, Zhi-Qiang
利用天然微量营养素预防癌症的表观遗传机制主要围绕植物提取物。然而，包括动物肽在内的大核素在癌症表观遗传修饰中的作用一直难以捉摸。在中国传统医学中，甲鱼作为一种功能性食物，通过未知的机制增强免疫力，历史悠久。本研究旨在探讨甲鱼肽对胃癌细胞microRNA（miRNA）表达的影响，并分析其潜在的抗胃癌作用机制。采用AffysseGeneChip miRNA 3.0 Array和定量聚合酶链反应检测甲鱼肽对人胃癌AGS细胞中miRNA表达的影响。结果表明，101个miRNA（49个上调的miRNA和52个下调的miRNA）在甲鱼肽处理后的AGS细胞中显著差异表达。几种抑癌miRNA表达显著上调，包括miRNA-375、let-7 d、miRNA-429、miRNA-148 a/148 b和miRNA-34 a。通路分析表明甲鱼肽可能通过Hippo信号通路和叉头盒O信号通路发挥抗癌作用。因此，这些结果表明甲鱼肽具有通过调节GC细胞中miRNA表达来影响癌症相关通路的能力。
Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long-history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft-shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft-shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft-shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA-375, let-7d, miRNA-429, miRNA-148a/148b and miRNA-34a. Pathway analysis indicated that soft-shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft-shelled turtle peptide has the capacity to influence cancer-related pathways through the regulation of miRNA expression in GC cells.