Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
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DOI：
10.1101/gr.126516.111
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发表时间：
2012-02-01
期刊：
影响因子：
7
通讯作者：
Holt, Robert A.
中科院分区：
文献类型：
--
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
据估计，全世界15%或更多的癌症负担可归因于已知的感染源。我们使用RNA-seq筛选结直肠癌和匹配正常组织标本，然后减去宿主序列，发现相对于对照标本，肿瘤中有明显的核梭杆菌序列。具核梭菌是一种侵袭性厌氧菌，以前曾被认为与牙周炎和阑尾炎有关，但与癌症无关。梭杆菌是粪便微生物群中罕见的成分，但以前从炎症肠道黏膜的活检中培养过。我们从冷冻肿瘤标本中分离出一株梭杆菌；这显示了最高的序列相似性已知的肠粘膜分离，并证实是侵入性的。我们通过对99名受试者的定量PCR分析，证实了肿瘤中梭杆菌序列比匹配的正常对照组织中多（p = 2.5 × 10(-6)），并且我们观察到与淋巴结转移呈正相关。
An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the fecal microbiota, but have been cultured previously from biopsies of inflamed gut mucosa. We obtained a Fusobacterium isolate from a frozen tumor specimen; this showed highest sequence similarity to a known gut mucosa isolate and was confirmed to be invasive. We verified overabundance of Fusobacterium sequences in tumor versus matched normal control tissue by quantitative PCR analysis from a total of 99 subjects (p = 2.5 x 10(-6)), and we observed a positive association with lymph node metastasis.