The COMPASS Subunit Spp1 Links Histone Methylation to Initiation of Meiotic Recombination
The COMPASS Subunit Spp1 Links Histone Methylation to Initiation of Meiotic Recombination
复制标题
DOI：
10.1126/science.1225739
复制
发表时间：
2013-01-11
期刊：
影响因子：
56.9
通讯作者：
Geli, Vincent
中科院分区：
文献类型：
--
作者：
Acquaviva, Laurent;Szekvoelgyi, Lorant;Geli, Vincent
在减数分裂期间，遗传性状的组合关联来自亲本染色体之间的同源重组。组蛋白H3赖氨酸4三甲基化标志着酵母和哺乳动物中的减数分裂重组热点，但这种无处不在的染色质修饰如何与依赖于Spo 11的双链断裂（DSB）的启动相关仍不清楚。在这里，我们表明，栓系的PHD含蛋白质，Spp 1（COMPASS复合物的一个组成部分），重组冷地区是足以诱导DSB的形成。此外，我们发现Spp1与Mer2（DSB形成所需的分化染色体轴的关键蛋白）发生物理相互作用。因此，通过与H3K4me3和Mer2相互作用，Spp 1促进潜在的减数分裂DSB位点向染色体轴的募集，允许Spo 11在附近的核小体耗尽区域裂解。
During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions.