Standardized atlas of the brain of the desert locust, Schistocerca gregaria
Standardized atlas of the brain of the desert locust, Schistocerca gregaria
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DOI：
10.1007/s00441-008-0620-x
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发表时间：
2008-07-01
影响因子：
3.6
通讯作者：
Homberg, Uwe
中科院分区：
文献类型：
--
作者：
Kurylas, Angela E.;Rohlfing, Torsten;Homberg, Uwe
为了理解神经元网络的连通性，我们应该在一个共同的框架下研究它们的组成神经元。由于生理特征和染色神经元的形态学数据通常来自不同的个体大脑，因此只能在虚拟标准化的大脑中进行，以补偿个体间的差异。沙漠蝗（Schistocerca gregaria）是一种广泛用于分析嗅觉和视觉信号处理、内分泌功能和控制运动输出的神经网络的昆虫。为了给该物种大脑的神经回路分析提供一个通用的多用户平台，我们制作了一个标准化的三维蝗虫大脑。利用蝗虫全脑的连续共聚焦图像重建了10个大脑的34个神经区。为了标准化，我们比较了两种不同的方法：一种是使用仿射变换的迭代形状平均（ISA）程序，然后是迭代非刚性配准；另一种是使用全局和局部刚性变换，然后是局部非刚性变换的虚拟昆虫脑（VIB）协议。这两种方法都产生了一个标准的大脑，但用于不同的应用。虽然VIB技术的目的是可视化输入大脑之间的解剖差异，但ISA方法的目的恰恰相反，即消除这种差异。一个新的单独标记的神经元，连接小脑到中脑和下脑，已经被记录到ISA图谱中，并证明了它对未来神经网络分析的有效性和准确性。蝗虫的标准大脑可以在http://www.3d-insectbrain.com上找到。
In order to understand the connectivity of neuronal networks, their constituent neurons should ideally be studied in a common framework. Since morphological data from physiologically characterized and stained neurons usually arise from different individual brains, this can only be performed in a virtual standardized brain that compensates for interindividual variability. The desert locust, Schistocerca gregaria, is an insect species used widely for the analysis of olfactory and visual signal processing, endocrine functions, and neural networks controlling motor output. To provide a common multi-user platform for neural circuit analysis in the brain of this species, we have generated a standardized three-dimensional brain of this locust. Serial confocal images from whole-mount locust brains were used to reconstruct 34 neuropil areas in ten brains. For standardization, we compared two different methods: an iterative shape-averaging (ISA) procedure by using affine transformations followed by iterative nonrigid registrations, and the Virtual Insect Brain (VIB) protocol by using global and local rigid transformations followed by local nonrigid transformations. Both methods generated a standard brain, but for different applications. Whereas the VIB technique was designed to visualize anatomical variability between the input brains, the purpose of the ISA method was the opposite, i.e., to remove this variability. A novel individually labeled neuron, connecting the lobula to the midbrain and deutocerebrum, has been registered into the ISA atlas and demonstrates its usefulness and accuracy for future analysis of neural networks. The locust standard brain is accessible at http://www.3d-insectbrain.com.