DISTRIBUTION OF mRNAs ENCODING CLASSICAL PROGESTIN RECEPTOR, PROGESTERONE MEMBRANE COMPONENTS 1 AND 2, SERPINE mRNA BINDING PROTEIN 1, AND PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBERS 7 AND 8 IN RAT FOREBRAIN
DISTRIBUTION OF mRNAs ENCODING CLASSICAL PROGESTIN RECEPTOR, PROGESTERONE MEMBRANE COMPONENTS 1 AND 2, SERPINE mRNA BINDING PROTEIN 1, AND PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBERS 7 AND 8 IN RAT FOREBRAIN
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DOI：
10.1016/j.neuroscience.2010.10.051
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发表时间：
2011-01-13
期刊：
影响因子：
3.3
通讯作者：
Petersen, S. L.
中科院分区：
文献类型：
--
作者：
Intlekofer, K. A.;Petersen, S. L.
一些证据表明，存在多种孕激素受体，这可能是孕酮在中枢神经系统中产生快速和延迟效应的原因。长期以来，这种延迟效应一直被归因于经典的孕激素受体(PGR)的激活。最近的研究发现了新的孕激素信号分子，可能与快速效应有关。这些受体包括孕激素受体膜组分1(PGRMC1)、Pgrmc2、孕激素和ADIPOQ受体7(Paqr7)和Paqr8。这些分子的功能在非神经组织中得到了广泛的研究，但在神经组织中还没有得到广泛的研究，部分原因是不清楚哪些分子在大脑中表达，以及它们在哪里表达。为了解决这些问题，我们用放射性标记的寡核苷酸探针进行原位杂交，比较了雌激素处理的雌性大鼠前脑组织中编码PGR、PGRMC1、Pgrmc2、Paqr7和Paqr8的mRNAs的分布。我们还检测了蛇类mRNA结合蛋白1(Serbp1)的分布，Serbp1可能是PGRMC1的结合伙伴。相邻脑切片的分析表明，编码PGR、PGRMC1、Pgrmc2和Serbp1的mRNAs在下丘脑几个对雌性生殖至关重要的核团中表达最高。而Paqr7和Paqr8在下丘脑中的表达较低且均一，在丘脑核团中的表达较丰富。这些可能的孕激素信号分子的神经解剖学分布表明，PGRMC1和Pgrmc2可能在神经内分泌功能中发挥作用，而Paqr7和Paqr8更有可能调节感觉和认知功能。(C)2011年IBRO。爱思唯尔有限公司出版。保留所有权利。
Several lines of evidence suggest the existence of multiple progestin receptors that may account for rapid and delayed effects of progesterone in the CNS. The delayed effects have been long attributed to activation of the classical progestin receptor (Pgr). Recent studies have discovered novel progestin signaling molecules that may be responsible for rapid effects. These include progesterone receptor membrane component 1 (Pgrmc1), Pgrmc2, progestin and adipoQ receptor 7 (Paqr7) and Paqr8. The functions of these molecules have been investigated extensively in non-neural, but not in neural tissues, partly because it is unclear which are expressed in the brain and where they are expressed. To address these issues, we compared the distributions of mRNAs encoding Pgr, Pgrmc1, Pgrmc2, Paqr7 and Paqr8 using in situ hybridization with radiolabeled oligodeoxynucleotidyl probes in forebrain tissues of estradiol-treated female rats. We also examined the distribution of serpine mRNA binding protein 1 (Serbp1), a putative binding partner of Pgrmc1. Analyses of adjacent brain sections showed that the highest expression of mRNAs encoding Pgr, Pgrmc1, Pgrmc2 and Serbp1 was detected in several hypothalamic nuclei important for female reproduction. In contrast, expression patterns of Paqr7 and Paqr8 were low and homogeneous in the hypothalamus, and more abundant in thalamic nuclei. The neuroanatomical distributions of these putative progestin signaling molecules suggest that Pgrmc1 and Pgrmc2 may play roles in neuroendocrine functions while Paqr7 and Paqr8 are more likely to regulate sensory and cognitive functions. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.