Reduced blinking and long-lasting fluorescence of single fluorophores coupling to silver nanoparticles
Reduced blinking and long-lasting fluorescence of single fluorophores coupling to silver nanoparticles
复制标题
DOI：
10.1021/la702673p
复制
发表时间：
2008-04-01
期刊：
影响因子：
3.9
通讯作者：
Lakowicz, Joseph R.
中科院分区：
文献类型：
--
作者：
Fu, Yi;Zhang, Jian;Lakowicz, Joseph R.
单个有机荧光团的荧光信号具有随机闪烁和不可逆光漂白的特征。Cy 5的光致闪烁已经对这种流行的近红外（NIR）探针在生物应用中造成了各种限制。在这里，我们表明，荧光团-金属纳米颗粒（NP）复合物大大抑制Cy 5闪烁，并显着减少光漂白事件。研究了单个Cy 5分子的闪烁行为，并比较了在不存在和存在银纳米结构的情况下。观察到单个Cy 5分子的关闭时间群体的幂律分布。比较平均关闭时间以评估银纳米颗粒的等离子体效应和三重态衰减速率。我们还证明了在银纳米颗粒存在下增强的光稳定性。结果表明，等离子体激元控制led荧光可以导致一种新的物理机制，以增强荧光强度，减少闪烁，并增加光稳定性。
The fluorescence signal of single organic fluorophores is characterized by random blinking and irreversible photobleaching. Photoiriduced blinking of Cy5 has posed various limitations of this popular near-infrared (NIR) probe in biological applications. Here we show that fluorophore-metal nanoparticle (NP) complexes greatly suppress Cy5 blinking and noticeably reduce photobleaching events. The blinking behavior of single Cy5 molecules was investigated and compared in the absence and the presence of silver nanostructures. A power-law distribution of off time population was observed for single Cy5 molecules. Average off times were compared to evaluate the plasmonic effect of silver nanoparticles oil the triplet decay rates. We furthermore demonstrate enhanced photostability in the presence of silver NPs. The results show that plasmonic-control led fluorescence can lead to a novel physical mechanism to enhance fluorescence intensity, reduce blinking, and increase photostability.