Optimized Liquid-Chromatographic Method for Fluorometric Determination of Urinary-AminoIevuIinic Acid in Workers Exposed to Lead
Optimized Liquid-Chromatographic Method for Fluorometric Determination of Urinary-AminoIevuIinic Acid in Workers Exposed to Lead
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荧光法测定铅作业工人尿氨基乙酰丙酸的优化液相色谱法
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Yuklo
中科院分区：
文献类型：
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作者：
Tomokunl;Masayoshl lchlba;Yuklo
建立了铅作业工人尿中氨基乙酰丙酸（ALA）的荧光测定方法。使用配备荧光HPLC监测器的高效液相色谱仪（HPLC）。ALA水溶液的检测限为20 g/L（100 L样品中ALA为15 pmol）。尿ALA浓度的线性范围为0.1 ~ 100 mg/L。25名铅作业工人尿ALA测定值与常规比色法测定值相关良好（r = 0.996）。本方法灵敏度高，可用于尿中ALA的微量测定。
We developed a fluorometric method for determining Saminolevulinic acid (ALA) in urine of lead workers. A highperformance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 g/L (15 pmol of ALA in the 100-L sample). The working linear range of urinary ALA concentration was 0.1 to 100 mg/L. In 25 lead-exposed workers, ALA values by the present method for urine correlated well with those obtained by a conventional colorimetric method (r = 0.996). The advantage of the present method for microdetermination of urinary ALA is its high sensitivity.