NUCLEAR MAGNETIC-RESONANCE STUDY OF ACETIC-ACID PERMEATION OF LARGE UNILAMELLAR VESICLE MEMBRANES
NUCLEAR MAGNETIC-RESONANCE STUDY OF ACETIC-ACID PERMEATION OF LARGE UNILAMELLAR VESICLE MEMBRANES
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DOI：
10.1016/s0006-3495(79)85154-1
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发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
PRESTEGARD, JH
中科院分区：
文献类型：
--
作者：
ALGER, JR;PRESTEGARD, JH
利用 NMR 表征伪平衡条件下流动的独特能力，研究了乙酸穿过大型单层磷脂囊泡膜的渗透。采用了两种类型的实验：总线形状分析和选择性群体转移。这些技术对 0.001-10.0 s 时间尺度的渗透敏感。渗透速率对 pH 值和乙酸浓度的依赖性表明，中性乙酸单体是主要的渗透物种类，渗透系数为 5±。 2.次。 10-4 厘米/秒。讨论了渗透机制和 NMR 方法的适用性。
The permeation of acetic acid through large unilamellar phospholipid vesicle membranes was investigated using the unique capability of NMR to characterize flow under pseudo-equilibrium conditions. Two types of experiments have been employed: total line shape analysis and selective population transfer. These techniques are sensitive to permeation on time scales ranging from 0.001-10.0 s. The permeation rate dependence on pH and acetic acid concentration indicates that the neutral acetic acid monomer is the dominant permeant species with a permeation coefficient of 5 .+-. 2 .times. 10-4 cm/s. Mechanisms of permeation and the applicability of NMR methodology are discussed.