The Effect of Irreversible Electroporation on the Femur: Experimental Study in a Rabbit Model.
The Effect of Irreversible Electroporation on the Femur: Experimental Study in a Rabbit Model.
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不可逆电穿孔对股骨的影响：兔模型的实验研究
DOI：
10.1038/srep18187
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发表时间：
2015-12-10
影响因子：
4.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Song Y;Zheng J;Yan M;Ding W;Xu K;Fan Q;Li Z
不可逆电穿孔（IRE）是一种新型消融方法，已在患有肺癌、前列腺癌、肾癌、肝癌、淋巴结癌和骶前癌的人体中进行了测试。作为一种新的非热疗法，使用IRE消融肌肉骨骼系统肿瘤可能会降低骨折的发生率。我们的目的是确定皮质骨的消融阈值，并评估中期和长期的愈合过程和力学性能的股骨在兔模型后IRE消融。皮质骨的消融阈值在1090 V/cm和1310 V/cm之间（120个脉冲）。IRE消融的股骨显示没有可检测到的骨折，但确实表现出恢复的迹象，包括成骨细胞再生，血管生成和骨重建。在消融区域，IRE后4周出现血运重建。成骨活性在IRE后8周达到峰值，并在12周时保持较高水平。IRE后4周机械强度短暂下降，但在8周内恢复正常水平。我们的实验表明，IRE消融保留了骨皮质的结构完整性，并且消融的骨能够快速再生。IRE可能为原位骨组织消融提供独特的前景，因为在IRE消融区域可以实现快速血运重建和主动成骨。
Irreversible electroporation (IRE) is a novel ablation method that has been tested in humans with lung, prostate, kidney, liver, lymph node and presacral cancers. As a new non-thermal treatment, the use of IRE to ablate tumors in the musculoskeletal system might reduce the incidence of fractures. We aimed to determine the ablation threshold of cortical bone and to evaluate the medium- and long-term healing process and mechanical properties of the femur in a rabbit model post-IRE ablation. The ablation threshold of cortical bone was between 1090 V/cm and 1310 V/cm (120 pulses). IRE-ablated femurs displayed no detectable fracture but did exhibit signs of recovery, including osteoblast regeneration, angiogenesis and bone remodeling. In the ablation area, revascularization appeared at 4 weeks post-IRE. Osteogenic activity peaked 8 weeks post-IRE and remained high at 12 weeks. The mechanical strength decreased briefly 4 weeks post-IRE but returned to normal levels within 8 weeks. Our experiment revealed that IRE ablation preserved the structural integrity of the bone cortex and the ablated bone was able to regenerate rapidly. IRE may hold unique promise forin situbone tissue ablation because rapid revascularization and active osteogenesis in the IRE ablation area are possible.