Fibronectin is more active than fibrin or fibrinogen in promoting Staphylococcus aureus adherence to inserted intravascular catheters.
Fibronectin is more active than fibrin or fibrinogen in promoting Staphylococcus aureus adherence to inserted intravascular catheters.
复制标题
纤连蛋白在促进金黄色葡萄球菌粘附于插入的血管内导管方面比纤维蛋白或纤维蛋白原更活跃。
DOI：
10.1093/infdis/167.3.633
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Lew,DP
中科院分区：
文献类型：
--
作者：
Vaudaux,P;Pittet,D;Haeberli,A;Lerch,PG;Morgenthaler,JJ;Proctor,RA;Waldvogel,FA;Lew,DP
为了进一步明确纤维蛋白（原）和纤连蛋白在金黄色葡萄球菌粘附中心静脉导管中的作用，前瞻性研究了这些蛋白吸附在插入住院患者的管路上的数量、化学完整性和生物活性。聚氨酯套管促进了S.金黄色聚氯乙烯（P<0.01）或希克曼（P<0.001）套管，并且含有最低量的免疫测定的纤维连接蛋白，但不含纤维蛋白（原）。纤维蛋白原表现出广泛的损失的粘附促进活动插入套管，这是有关其蛋白水解分解，检测到的SDS-PAGE和免疫印迹与抗纤维蛋白原抗体，并证实了在体外研究与纯化的蛋白片段。相反，无论是完整的或片段化的纤连蛋白，虽然存在的量比纤维蛋白（原）低得多，可以积极促进。金黄色粘附在静脉导管上。
To further define the role of fibrin(ogen) and fibronectin inStaphylococcus aureusadherence to central venous catheters, the amount, chemical integrity, and biologic activity of these proteins adsorbed on lines inserted in hospitalized patients were prospectively studied. Polyurethane cannulas promoted a significantly lower adherence ofS. aureusthan polyvinyl chloride (P< .01) or Hickman (P< .001) cannulas and contained the lowest amount of immunologically assayed fibronectin but not of fibrin(ogen). Fibrinogen showed an extensive loss of adherence-promoting activity on inserted cannulas, which was related to its proteolytic breakdown, as detected by SDS-PAGE and immunoblots with antifibrinogen antibodies and confirmed by in vitro studies with purified protein fragments. In contrast, either intact or fragmented fibronectin, although present in much lower amounts than fibrin(ogen), could actively promoteS. aureusadherence onto intravenous catheters.