Influence of follicular health on the steroidogenic and morphological characteristics of bovine granulosa cells in vitro.
Influence of follicular health on the steroidogenic and morphological characteristics of bovine granulosa cells in vitro.
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卵泡健康对体外牛颗粒细胞类固醇生成和形态特征的影响。
DOI：
10.1530/jrf.0.0790185
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
M. Prisk
中科院分区：
文献类型：
--
作者：
K. Henderson;K. McNatty;P. Smith;M. Gibb;L. O’Keeffe;S. Lun;D. Heath;M. Prisk
在24小时的培养中，非闭锁卵泡细胞的类固醇产量随着卵泡直径的增加而增加。与非闭锁卵泡细胞相比，来自各种大小的闭锁卵泡的细胞产生的雌二醇-17 β含量较低，但孕酮含量很高。将培养时间延长至72 h后，闭锁卵泡颗粒细胞的每日孕酮和雌二醇-17 β产量变化不大。相反，在非闭锁卵泡细胞中，每日孕酮产量增加，每日雌二醇-17 β产量下降到闭锁卵泡细胞的水平。二丁基环AMP （1.0 mM）显著刺激闭锁卵泡细胞产生孕酮，而非闭锁卵泡细胞则没有。睾酮（1微克/毫升）对闭锁卵泡细胞产生孕酮没有影响，而雌二醇-17 β、雌酮、睾酮、雄烯二酮和5 α -二氢睾酮（0-1000 ng/ml）均显著抑制非闭锁卵泡细胞产生孕酮，且呈剂量依赖性。形态计量学分析显示，非闭锁和闭锁卵泡细胞之间的亚细胞差异很少。来自闭锁卵泡的细胞的平均细胞体积明显高于非闭锁卵泡，但主要亚细胞成分的平均体积不受卵泡健康的影响。闭锁卵泡细胞的浆、核膜和颗粒内质网的平均表面积也明显高于非闭锁卵泡细胞。
In 24-h cultures, steroid production by cells from non-atretic follicles increased with increasing follicular diameter. Cells from atretic follicles, of all sizes, produced low amounts of oestradiol-17 beta, but very high amounts of progesterone, relative to cells from non-atretic follicles. Increasing the culture period to 72 h caused little change in daily progesterone and oestradiol-17 beta production by granulosa cells from atretic follicles. In contrast, in cells from non-atretic follicles, daily progesterone production increased and daily oestradiol-17 beta production decreased to the levels observed with cells from atretic follicles. Dibutyryl cyclic AMP (1.0 mM) significantly stimulated progesterone production by cells from atretic, but not from non-atretic, follicles. Testosterone (1 microgram/ml) had no effect on progesterone production by cells from atretic follicles, while oestradiol-17 beta, oestrone, testosterone, androstenedione and 5 alpha-dihydro-testosterone (0-1000 ng/ml) each significantly suppressed progesterone production by cells from non-atretic follicles in a dose-dependent manner. Morphometric analysis revealed few subcellular differences between cells from non-atretic and atretic follicles. Mean cell volume was significantly higher for cells from atretic compared to non-atretic follicles, but the mean volumes of the major subcellular components were not influenced by follicle health. The mean surface area of the plasma and nuclear membrane, and granular endoplasmic reticulum was also significantly higher in cells from atretic compared to non-atretic follicles.