Polarization properties of fluorescent BSA protected Au25 nanoclusters.
Polarization properties of fluorescent BSA protected Au25 nanoclusters.
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DOI：
10.1039/c3nr34152f
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发表时间：
2013-04-21
期刊：
影响因子：
6.7
通讯作者：
Gryczynski I
中科院分区：
文献类型：
--
作者：
Raut S;Chib R;Rich R;Shumilov D;Gryczynski Z;Gryczynski I
牛血清白蛋白保护的金纳米团簇(AU25)由于其独特的光谱性质和在生物物理中的潜在应用而引起了人们的极大关注。虽然有关于合成策略、光谱和应用的报道，但对它们的偏振行为知之甚少。在这项研究中，我们合成了BSA保护的AU25纳米团簇，并研究了它们的稳态和时间分辨荧光性质，包括在不同溶剂：甘油、丙二醇和水中的偏振行为。我们证明了通过减去瑞利散射可以将纳米团簇的吸收从消光光谱中分离出来。纳米团簇的吸收光谱可以用三个高斯分量很好地近似。通过比较BSA AU25团簇和罗丹明B在水中的发射，我们估计纳米团簇的量子产额高于0.06。BSAAU25团簇的荧光寿命较长且不均匀，S的平均荧光寿命为1.84μ，而甘油在−20°C时的各向异性很高，达到0.35.然而，激发各向异性强烈地依赖于激发波长，这表明不同的跃迁矩有显著的重叠。在水中的各向异性衰减显示关联时间小于0.2μ，S，在丙二醇中测得的关联时间较长，初始各向异性依赖于激发波长。BSA AU25簇具有长寿命和高极化的特点，在研究大分子如大分子蛋白质复合体等方面具有潜在的应用前景。
BSA protected gold nanoclusters (Au25) are attracting great deal of attention due to their unique spectroscopic properties and possible use in biophysical applications. Although there are reports on synthetic strategies, spectroscopy and applications, little is known about their polarization behavior. In this study, we synthesized the BSA protected Au25 nanoclusters and studied their steady state and time resolved fluorescence properties including polarization behavior in different solvents: glycerol, propylene glycol and water. We demonstrated that the nanocluster absorption can be separated from the extinction spectrum by subtraction of Rayleigh scattering. The nanocluster absorption spectrum is well approximated by three Gaussian components. By a comparison of the emissions from BSA Au25 clusters and rhodamine B in water, we estimated the quantum yield of nanoclusters to be higher than 0.06. The fluorescence lifetime of the BSA Au25 cluster is long and heterogeneous with an average value of 1.84 μs. In glycerol at −20°C the anisotropy is high, reaching a value of 0.35. However, the excitation anisotropy strongly depends on the excitation wavelengths indicating a significant overlap of the different transition moments. The anisotropy decay in water reveals a correlation time below 0.2 μs. In propylene glycol the measured correlation time is longer and initial anisotropy depends on the excitation wavelength. The BSA Au25 cluster, due to long lifetime and high polarization, can potentially be used in studying large macromolecules such as protein complexes with large molecular weight.
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