ARCHITECTURAL ORGANIZATION IN THE INTERPHASE NUCLEUS OF THE PROTOZOAN TRYPANOSOMA-BRUCEI - LOCATION OF TELOMERES AND MINICHROMOSOMES
ARCHITECTURAL ORGANIZATION IN THE INTERPHASE NUCLEUS OF THE PROTOZOAN TRYPANOSOMA-BRUCEI - LOCATION OF TELOMERES AND MINICHROMOSOMES
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DOI：
10.1002/j.1460-2075.1990.tb07443.x
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发表时间：
1990-08-01
期刊：
影响因子：
11.4
通讯作者：
VANDERPLOEG, LHT
中科院分区：
文献类型：
--
作者：
CHUNG, HMM;SHEA, C;VANDERPLOEG, LHT
我们研究了染色质的空间组织在间期G1，S和G2细胞核的原生动物布氏锥虫，应用原位杂交与传统的荧光和共聚焦扫描光学显微镜。大多数的锥虫端粒GGGTTA重复从不同的染色体被发现聚集在一起，通过核内部或定位在核周边的网络中延伸。100条微型染色体的群体通常不对称地定位：要么聚集在与核膜密切相关的窄带中，要么分布成几个簇，分离成大约一半的细胞核。细胞核组织在细胞周期和发育过程中可能会发生变化。我们的结论是，非随机空间定位的DNA存在于这种原生动物的细胞核。在布氏锥虫间期核中发现高水平的结构组织是理解染色体结构和功能及其在基因表达控制中的作用的重要的第一步。
We studied the spatial organization of chromatin in the interphase G1, S and G2 nucleus of the protozoan Trypanosoma brucei, applying in situ hybridization with conventional fluorescence and confocal scanning optical microscopy. The majority of the trypanosome telomere GGGTTA repeats from different chromosomes were found clustered together, either extending in a network through the nuclear interior or localized at the nuclear periphery. The population of one hundred mini‐chromosomes was often asymmetrically located: either clustered in a narrow band in close association with the nuclear envelope or distributed into several clusters that segregated into roughly one half of the nucleus. The nuclear organization may undergo modifications during the cell cycle and development. We conclude that non‐random spatial positioning of DNA exists in the nucleus of this protozoan. Finding a high level of structural organization in the interphase nucleus of T.brucei is an important first step towards understanding chromosome structure and functioning and its role in the control of gene expression.