A needle-type optical enzyme sensor system for determining glucose levels in fish blood
A needle-type optical enzyme sensor system for determining glucose levels in fish blood
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DOI：
10.1016/j.aca.2006.04.068
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发表时间：
2006-07-28
影响因子：
6.2
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Endo, Hideaki;Yonemori, Yuki;Mitsubayashi, Kohji
建立了一种快速、简便测定鱼血中葡萄糖含量的针式生物传感器系统。该传感器由针型空心容器（18号针）、固定化酶膜和钌配合物光纤探针组成。以葡萄糖氧化酶、含叠氮单元的水溶性光致聚合物和超薄透析膜为原料制备酶膜。将光纤探针插入放置在针型中空容器中的卷起的酶膜中。鱼血浆中葡萄糖水平的校准曲线呈线性。一次测定在3 min内完成，重复性好，可重复测定60次，无需更换酶膜。将传感器插入鱼的尾静脉以测量血糖水平。探头在插入后立即响应，可以监测葡萄糖水平。在48-157 mg dl（-1）范围内，使用传感器和常规方法测定的值之间观察到良好的相关性（相关系数为0.9474）。(c)2006 Elsevier B. V.保留所有权利。
A needle-type biosensor system was developed for rapid and simple determination of glucose levels in fish blood. The sensor comprises a needle-type hollow container (18-gauge needle), immobilized enzyme membrane and optic fiber probe with ruthenium complex. The enzyme membrane was prepared from glucose oxidase, azide-unit pendant water-soluble photopolymer and an ultra-thin dialysis membrane. The optic fiber probe was inserted into the rolled enzyme membrane placed in the needle-type hollow container. The calibration curve was linear for glucose levels in fish plasma. One assay was completed within 3 min. A good reproducibility was observed for 60 times without exchange of the enzyme membrane. The sensor was inserted into the caudal vein of fish to measure blood glucose levels. The sensor responded immediately after insertion and glucose levels could be monitored. Good correlations were observed between values determined using the sensor and conventional methods in the range of 48-157 mg dl(-1) (correlation coefficient, 0.9474). (c) 2006 Elsevier B.V. All rights reserved.