Diagnosis of Eimeria species using traditional and molecular methods in field studies
Diagnosis of Eimeria species using traditional and molecular methods in field studies
复制标题
DOI：
10.1016/j.vetpar.2010.11.015
复制
发表时间：
2011-03-10
影响因子：
2.6
通讯作者：
Albuquerque, George Rego
中科院分区：
文献类型：
--
作者：
Carvalho, Fabio Santos;Wenceslau, Amauri Arias;Albuquerque, George Rego
本研究的目的是鉴定和表征种艾美耳球虫在肉鸡使用传统的形态和病理加上分子（DNA扩增）诊断方法。使用这些技术的组合，有可能确定鸡群中存在多种循环物种，以及其中一些物种的较高频率，特别是在100%的鸡群中确定的早熟艾美耳球虫和巨型艾美耳球虫。其它种类的分布频率依次为缓艾美耳球虫和毒害艾美耳球虫（93.3%）、柔嫩艾美耳球虫（76.7%）、堆型艾美耳球虫（56.7%）和布氏艾美耳球虫（16.7%）。然而，使用病变评分，最常见的物种是E。maxima（46.7%），E. acervulina（30%）、E. tenella（23.3%）和E. necatrix（10%）。E. brunetti和E.用病变评分法未发现早发。DNA扩增对至少20个卵囊的现场样品中的艾美耳球虫种具有检测灵敏度。DNA扩增作为常规诊断技术在禽舍的实施可以帮助艾美耳球虫种群。(C)2010 Elsevier B.V.保留所有权利。
The objective of this study was to identify and characterize species of Eimeria in broiler chickens using traditional morphological and pathological plus molecular (DNA amplification) diagnostic methodologies. Using a combination of those techniques it was possible to identify the presence of multiple circulating species in the flock as well as higher frequencies for some of them, especially Eimeria praecox and Eimeria maxima, which were identified in 100% of the flocks. The frequencies of the other species were Eimeria mitis and Eimeria necatrix (93.3%), Eimeria tenella (76,7%), Eimeria acervulina (56.7%) and Eimeria brunetti (16.7%). However using the lesion score, the most common species were E. maxima (46.7%), E. acervulina (30%), E. tenella (23.3%), and E. necatrix (10%). E. brunetti and E. praecox were not identified by using lesion score. DNA amplification had detection sensitivity for Eimeria species in the field samples of at least 20 oocysts. The implementation of DNA amplification as a routine diagnostic technique in aviaries can assist Eimeria population. (C) 2010 Elsevier B.V. All rights reserved.