Effect of Drought Stress on Total Phenolic, Lipid Peroxidation, and Antioxidant Activity of Achillea Species
Effect of Drought Stress on Total Phenolic, Lipid Peroxidation, and Antioxidant Activity of Achillea Species
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DOI：
10.1007/s12010-015-1909-3
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发表时间：
2016-02-01
影响因子：
3
通讯作者：
Goli, Sayed Amir Hossein
中科院分区：
文献类型：
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作者：
Gharibi, Shima;Tabatabaei, Badraldin Ebrahim Sayed;Goli, Sayed Amir Hossein
采用3种模型系统研究了4种不同灌溉方式(100%田间持水量正常灌溉、75%轻度胁迫、50%中度胁迫和25%重度胁迫)下三种阿基利木(Achilea millefolium、A.nobilis和A.filipendulina)的总酚含量(TPC)、总黄酮含量(TFC)、脯氨酸(Pro)、丙二醛(MDA)、过氧化氢(H_2O_2)和抗氧化活性的变化。在中度和重度胁迫条件下，总生物量(TPC)和总生物量(TFC)最高，分别为47.13 mg单宁酸/gDW和20.86 mg栎素/gW。在2，2-二苯基-1-苦基肼(DPPH)测定中，小叶沙打旺的抗氧化活性最高(70.28%)，细叶沙打旺的抗氧化活性最低(53.21%)，而在FTC模型体系中，在严重干旱条件下，黄花沙打旺的抗氧化活性最高(1.934)。在亚油酸模型体系中，在低干旱胁迫条件下，紫云英的抗氧化活性最高。轻度干旱胁迫(75%FC)和中度干旱胁迫(50%FC)均增加了叶片的丙二醛和过氧化氢含量，但重度干旱胁迫(25%FC)降低了丙二醛和过氧化氢的含量。此外，小叶沙打旺的过氧化氢含量最低(4.96 nm/gFW)，丙二醛含量最低(176.32µm ol/g)。不同代谢物之间的关系研究表明，TPC和TFC呈显著正相关。结果表明，中度干旱胁迫(50%FC)可获得较高的TPC和TFC，而重度干旱胁迫(25%FC)具有最高的抗氧化活性。
The changes in total phenolic content (TPC), total flavonoid content (TFC), proline, malondialdehyde (MDA), H2O2, and antioxidant activity were assessed based on three model systems in three Achillea species (Achillea millefolium, A. nobilis, and A. filipendulina) growing under four irrigation regimes, including 100 % FC (field capacity as normal irrigation) 75 % FC (low stress), 50 % FC (moderate stress), and 25 % FC (severe stress) conditions. The highest TPC (47.13 mg tannic acid/g DW) and TFC (20.86 mg quercetin/g W) were obtained in A. filipendulina under moderate and severe stress conditions. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the highest and the lowest antioxidant activity was obtained for A. millefolium (70.28 %) and A. filipendulina (53.21 %), respectively, while in the FTC model system A. nobilis revealed the highest antioxidant activity (1.934) in severe drought condition. In the linoleic model system, the highest antioxidant activity was observed under low drought stress condition in A. nobilis. MDA and H2O2 content were increased due to both low (75 % FC) and moderate (50 % FC) drought stress, but they were decreased under severe stress condition (25 % FC). Furthermore, A. millefolium revealed the lowest H2O2 (4.96 nm/g FW) and MDA content (176.32 mu mol/g). Investigation of the relationship among different metabolites showed a strong positive correlation with TPC and TFC. Finally, the moderate drought stress treatment (50 % FC) was introduced as the optimum condition to obtain appreciable TPC and TFC,, while the highest antioxidant activity was obtained in severe stress condition (25%FC).