Sterol composition of dinoflagellates: Different abundance and composition in heterotrophic species and resting cysts
Sterol composition of dinoflagellates: Different abundance and composition in heterotrophic species and resting cysts
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DOI：
10.2343/geochemj.1.0063
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发表时间：
2010-01-01
影响因子：
0.8
通讯作者：
Horiguchi, Takeo
中科院分区：
文献类型：
--
作者：
Amo, Miki;Suzuki, Noriyuki;Horiguchi, Takeo
自养甲藻Periddium umbonatumvar.Iaequale、Akashiwo sanguia、Scrippsiella tincVictoria和Prorocentum Micans通常含有五种主要的甾醇：胆固醇、4-甲基胆烷-3-醇、4，24-二甲基胆甾烷-3-醇、地诺甾醇和地诺甾醇。异养沟鞭藻Protperiddium crassipe的游动细胞主要含有胆固醇、4，24-二甲基胆烷-3-醇、地甾醇、地诺甾醇和4-四甲基胆烷-3-醇等主要游离甾醇。异养甲藻的恐甾醇含量约为自养甲藻的4-12倍，这表明异养甲藻是某些底泥中地甾醇的重要来源。4-四甲基胆烷-3-醇在其他异养甲藻的培养样品中未见报道。异养甲藻中4-甲基甾醇组成的差异可能与培养实验所使用的饲料有关。云杉休眠孢囊是一种典型的变种。其主要游离态为4-甲基胆烷-3-醇、4，24-二甲基胆甾烷-3-醇、地诺甾醇和C-31-Delta(5.22)-甾醇。在甲藻活动细胞的培养样本中尚未发现C-31 Delta(5.22)-甾醇。这种化合物可能是在休眠阶段产生的，因此可能成为甲藻休眠包囊的潜在生物标志物。
The motile cells of the autotrophic dinoflagellates Peridinium umbonatum var. inaequale, Akashiwo sanguinea, Scrippsiella tinctoria, and Prorocentrum micans commonly contain five major sterols: cholesterol, 4-methylcholestan-3-ol, 4,24-dimethylcholestan-3-ol, dinosterol, and dinostanol. The motile cell of the heterotrophic dinoflagellate Protoperidinium crassipes contains cholesterol, 4,24-dimethylcholestan-3-ol, dinosterol, dinostanol, and 4-tetramethylcholestan-3-ol as major free sterols. The dinosterol concentrations of heterotrophic dinoflagellates are about 4-12 times higher than those of autotrophic species, suggesting that the heterotrophic dinoflagellate is an important source of dinosterol in some sediments. 4-Tetramethylcholestan-3-ol has not been reported in cultured samples of other heterotrophic dinoflagellates. The difference in 4-methyl sterol composition in heterotrophic dinoflagellates may be related to the feeds used during the culture experiment. The resting cyst of P. umbonatum var. inaequale contains 4-methylcholestan-3-ol, 4,24-dimethylcholestan-3-ol, dinosterol, dinostanol, and C-31 Delta(5.22)-sterol as major free sterols. The C-31 Delta(5.22)-sterol has not been reported in cultured samples of motile cells from dinoflagellates. This compound might be produced during the resting stage, and thus may serve as a potential biomarker for the resting cysts of dinoflagellates.