von Willebrand Factor and Prekallikrein in Plasma Are Associated With Thrombus Volume in Abdominal Aortic Aneurysms
von Willebrand Factor and Prekallikrein in Plasma Are Associated With Thrombus Volume in Abdominal Aortic Aneurysms
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DOI：
10.1177/1538574416666224
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发表时间：
2016-08-01
影响因子：
0.9
通讯作者：
Eiberg, Jonas P.
中科院分区：
文献类型：
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作者：
Ghulam, Qasam M.;Bredahl, Kim K.;Eiberg, Jonas P.
目的：内皮细胞衬里的破坏可能是连接腔内血栓和腹主动脉瘤生长的事件之一。在本研究中，我们检测了血浆中血管性血友病因子活性、凝血接触蛋白和炎症生物标志物是否与管腔内血栓体积相关，以寻找内皮损伤和血栓大小的生化标志物。设计：前瞻性研究，将潜在的内皮生物标志物与计算机断层扫描血管造影获得的管腔内血栓体积相关。材料和方法：连续从38例无症状肾下腹主动脉瘤患者中采集血浆。在自动化和内部平台上测量血浆中的血管性血友病因子活性、凝血酶生成时间、因子XII和前激肽释放酶浓度。共有8例患者因持续抗凝治疗、肾损害或未出现而被排除，因此留下30例患者进行进一步分析。所有患者均行CT血管造影，OsiriX 6.5离线定量管腔内血栓体积。结果：管腔内血栓体积中位数为42.7 mL。斯皮尔曼相关分析显示，血栓体积、血管性血友病因子活性（= 0.56，P =.0013），血浆中前激肽释放酶浓度（= 0.54，P =.002）。结论：血管性血友病因子活性和前激肽释放酶的浓度可能对腹主动脉瘤血栓的演变和可能的生物标志物都很重要。动脉瘤生长
Objectives: Disruption of the endothelial lining may be one of the events linking intraluminal thrombus and abdominal aortic aneurysm growth. In the present study, we examined whether von Willebrand factor activity in plasma, contact proteins of blood coagulation, and inflammatory biomarkers may be associated with intraluminal thrombus volume in search of a biochemical marker of endothelial damage and thrombus size.Design: Prospective study, correlating potential endothelial biomarkers and intraluminal thrombus volume acquired by computed tomography angiography.Materials and Methods: Plasma was consecutively obtained from 38 patients with asymptomatic infrarenal abdominal aortic aneurysm. von Willebrand factor activity, thrombin generation time, factor XII, and prekallikrein concentration were measured in plasma on automated and in-house platforms. In total, 8 patients were excluded due to ongoing anticoagulant therapy, renal impairment, or nonappearance, thus leaving 30 patients for further analysis. All patients had computed tomography angiography, and intraluminal volume was quantified off-line by OsiriX 6.5.Results: Median intraluminal thrombus volume was 42.7 mL. Spearman correlation analysis revealed a positive correlation between thrombus volume, von Willebrand factor activity ( = 0.56, P = .0013), and prekallikrein concentration in plasma ( = 0.54, P = .002).Conclusion: von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth.