A Test of Seven Candidate Barcode Regions from the Plastome in Picea (Pinaceae)
A Test of Seven Candidate Barcode Regions from the Plastome in Picea (Pinaceae)
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云杉（松科）质体七个候选条形码区域的测试
DOI：
10.1111/j.1744-7909.2010.00995.x
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发表时间：
2010-12-01
影响因子：
11.4
通讯作者：
Wang, Xiao-Quan
中科院分区：
文献类型：
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作者：
Ran, Jin-Hua;Wang, Pei-Pei;Wang, Xiao-Quan
DNA条形码作为物种区分的工具，已在动物、藻类和真菌中得到有效应用，但对于哪些DNA区域可以用作陆地植物的标准条形码仍存在争议。裸子植物，尤其是针叶树，是森林的重要组成部分，由于其野外采集频率高，迫切需要通过DNA条形码对其进行鉴定。然而，DNA条形码在裸子植物中的可行性还没有被检查的基础上，一个密集的物种采样。在这里，我们从质体组中选择了七个候选DNA条形码（matK，rbcL，rpoB，rpoC1，atpF-atpH，psbA-trnH和psbK-psbI）来评估它们在云杉中的适用性。结果表明，尽管matK + rbcL可以作为云杉的双位点条形码，但它们或它们的不同组合对云杉物种都没有足够的分辨率。这些候选条形码在云杉中的低效率可能是由于叶绿体基因组的父系遗传、世代时间长、最近的辐射以及风媒辅助下的频繁种间杂交造成的。这些因素中的一些也可能是造成其他植物类群条形码化困难的原因。此外，核LEAFY基因作为陆地植物条形码的潜力进行了讨论。
DNA barcoding, as a tool for species discrimination, has been used efficiently in animals, algae and fungi, but there are still debates on which DNA region(s) can be used as the standard barcode(s) for land plants. Gymnosperms, especially conifers, are important components of forests, and there is an urgent need for them to be identified through DNA barcoding because of their high frequency of collection in the field. However, the feasibility of DNA barcoding in gymnosperms has not been examined based on a dense species sampling. Here we selected seven candidate DNA barcodes from the plastome (matK, rbcL, rpoB, rpoC1, atpF-atpH, psbA-trnH, and psbK-psbI) to evaluate their suitability in Picea (spruce). The results showed that none of them or their different combinations has sufficient resolution for spruce species, although matK+rbcL might be used as a two-locus barcode. The low efficiency of these candidate barcodes in Picea might be caused by the paternal inheritance of the chloroplast genome, long generation time, recent radiation, and frequent inter-specific hybridization aided by wind pollination. Some of these factors could also be responsible for the difficulties in barcoding other plant groups. Furthermore, the potential of the nuclear LEAFY gene as a land plant barcode was discussed.