Dry Eye: A Protein Conformational Disease
Dry Eye: A Protein Conformational Disease
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DOI：
10.1167/iovs.14-15992
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发表时间：
2015-03-01
影响因子：
4.4
通讯作者：
Raja, Sufi O.
中科院分区：
文献类型：
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作者：
Azharuddin, Mohammad;Khandelwal, Jayesh;Raja, Sufi O.
目的.本研究的目的是确定缺水性干眼（ADDE）是否是一种蛋白质构象疾病。到目前为止，治疗方案一直是基于经验的结果，但这些观察可能揭示新的治疗诊断方法ADDE管理。招募50例ADDE患者和46例健康志愿者。对受试者进行泪液分泌试验、泪膜破裂时间、泪半月板高度和荧光素染色试验。收集ADDE和对照患者的泪液蛋白，并使用Schirmer条带提取。蛋白质聚集是通过评估平均蛋白质大小，使用动态光散射（DLS），快速高效液相色谱（FPLC），同步荧光光谱（SFS）。动态光散射数据显示，相对较高的丰度聚集蛋白在ADDE患者比对照组。对于对照组，泪液蛋白的大小分布是
PURPOSE. The purpose of this study was to determine whether aqueous-deficient dry eyes (ADDE) is a protein conformational disease. Up to now the therapeutic regimen has been based on empirical results, but these observations may unfold new theranostic approaches for ADDE management.METHODS. Fifty ADDE patients and 46 healthy volunteers were recruited. Schirmer's test, tear breakup time, tear meniscus height, and fluorescein staining tests were conducted on the subjects. Tear protein for ADDE and control patients was collected and extracted using Schirmer's strip. Protein aggregation was studied by appraisal of average protein size, using dynamic light scattering (DLS), fast performance liquid chromatography (FPLC), and synchronous fluorescence spectroscopy (SFS).RESULTS. Dynamic light scattering data showed a comparatively higher abundance of aggregated proteins in ADDE patients than that in controls. For controls, the size distribution of tear proteins was