Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry
Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry
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DOI：
10.1016/j.jchromb.2004.12.017
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发表时间：
2005-03-25
影响因子：
3
通讯作者：
Ikeda, N
中科院分区：
文献类型：
--
作者：
Kage, S;Kudo, K;Ikeda, N
建立了一种灵敏、简便的气相色谱-质谱法测定人全血和尿液中溴离子的方法。溴在丙酮和磷酸盐缓冲液（pH 6.8）的混合物中与对甲苯磺酸五氟苄基酯烷基化。使用气相色谱-质谱法与正离子EI模式分析得到的衍生物。该化合物的检测下限为1 mg/l。溴化物的校准曲线在2 - 100 mg/l的浓度范围内呈线性。应用该法测定了吸入性溴化烃中毒患者全血和尿液中溴离子的含量。(C)2004 Elsevier B. V.保留所有权利。
We devised a sensitive and simple method for determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. Bromide was alkylated with pentafluorobenzyl p-toluenesulphonate in the mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed using gas chromatography-mass spectrometry with the positive-ion El mode. The lower limit of detection for the compound was 1 mg/l. The calibration curve for bromide was linear over the concentration range from 2 to 100 mg/l. With use of this method, levels of bromide in whole blood and urine were determined in cases of poisoning by inhaled brominated hydrocarbons. (C) 2004 Elsevier B.V. All rights reserved.