Increased expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in kidney and bladder carcinomas.
Increased expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in kidney and bladder carcinomas.
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发表时间：
1993-11
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通讯作者：
L. Brown;B. Berse;R. Jackman;K. Tognazzi;E. Manseau;H. Dvorak;D. Senger
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作者：
L. Brown;B. Berse;R. Jackman;K. Tognazzi;E. Manseau;H. Dvorak;D. Senger
血管通透性因子（VPF），也称为血管内皮生长因子，是一种与肿瘤相关的微血管通透性过高和血管生成有关的分泌蛋白。通过原位杂交，12 例肾细胞癌中有 11 例的肿瘤细胞表达高水平的 VPF 信使 RNA (mRNA)，唯一的例外是相对无血管的乳头状变体。邻近坏死区域的表达进一步增强。通过免疫组织化学，肿瘤细胞和肿瘤附近小血管中的内皮细胞对 VPF 蛋白均进行了强烈染色。内皮细胞不表达可检测到的 VPF mRNA，但表达高水平的 VPF 受体 flt-1 和 KDR mRNA，表明内皮细胞染色可能反映了邻近肿瘤细胞分泌的 VPF 的结合。三种移行细胞癌也对 VPF mRNA 进行了强烈标记。这些数据表明 VPF 在这两种常见人类恶性肿瘤的血管生物学中发挥着重要作用。
Vascular permeability factor (VPF), also known as vascular endothelial growth factor, is a secreted protein implicated in tumor-associated microvascular hyperpermeability and angiogenesis. Tumor cells in 11 of 12 renal cell carcinomas expressed high levels of VPF messenger RNA (mRNA) by in situ hybridization, the only exception being a case of the relatively avascular papillary variant. Expression was further accentuated adjacent to areas of necrosis. Both tumor cells and endothelial cells in small vessels adjacent to tumor stained strongly for VPF protein by immunohistochemistry. Endothelial cells did not express detectable VPF mRNA, but did express high levels of mRNA for the VPF receptors flt-1 and KDR indicating that the endothelial cell staining likely reflects binding of VPF secreted by adjacent tumor cells. Three transitional cell carcinomas also labeled strongly for VPF mRNA. These data suggest an important role for VPF in the vascular biology of these two common human malignancies.