PEPTIDOGLYCAN SYNTHESIS DURING THE CELL-CYCLE OF ESCHERICHIA-COLI - COMPOSITION AND MODE OF INSERTION
PEPTIDOGLYCAN SYNTHESIS DURING THE CELL-CYCLE OF ESCHERICHIA-COLI - COMPOSITION AND MODE OF INSERTION
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DOI：
10.1128/jb.171.11.5783-5794.1989
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发表时间：
1989-11-01
影响因子：
3.2
通讯作者：
NANNINGA, N
中科院分区：
文献类型：
--
作者：
DEJONGE, BLM;WIENTJES, FB;NANNINGA, N
对大肠杆菌细胞周期合成的肽聚糖的组成和插入方式进行了测定。这是对在同步生长的培养中定期脉冲标记的肽聚糖进行的。脉冲标记的(新合成的)肽聚糖的化学成分在整个细胞周期中保持不变，这是通过对多肽成分的高压液相色谱分析判断的。根据双二糖四糖化合物的受体-供体放射性比推测了其插入方式。这一比例在伸长细胞中较低，在收缩细胞中较高。这表明在伸长过程中，肽聚糖是以单链形式插入的，而在收缩过程中，则发生了多链(或连续的单链)插入。用FTSA分区突变体进行的实验表明，新合成的肽聚糖的组成和插入方式在整个缩合过程中保持不变。我们的结果表明，从细胞伸长到极帽形成的转变可能是肽聚糖插入方式的改变，而不是化学结构的变化。
The composition and the mode of insertion of peptidoglycan synthesized during the cell cycle of Escherichia coli were determined. This was carried out on peptidoglycan that was periodically pulse-labeled in synchronously growing cultures. The chemical composition of the pulse-labeled (newly synthesized) peptidoglycan remained constant throughout the cell cycle, as judged from high-pressure liquid chromatography analysis of the muropeptide composition. The mode of insertion was deduced from the acceptor-donor radioactivity ratio in the bis-disaccharide tetratetra compound. The ratio was low in elongating cells and high in constricting cells. This indicates that during elongation, peptidoglycan was inserted as single strands, whereas during constriction, a multistranded (or sequential single-stranded) insertion occurred. Experiments with an ftsA division mutant suggested that the composition and mode of insertion of newly synthesized peptidoglycan remained the same throughout the constriction process. Our results imply that the changed mode of insertion rather than the chemical structure of the peptidoglycan might be responsible for the transition from cell elongation to polar cap formation.