Main Ustilaginoidins and Their Distribution in Rice False Smut Balls.
Main Ustilaginoidins and Their Distribution in Rice False Smut Balls.
复制标题
稻曲霉中主要乌斯蒂菌素及其分布
DOI：
10.3390/toxins7104023
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发表时间：
2015-10-09
期刊：
影响因子：
4.2
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Meng J;Sun W;Mao Z;Xu D;Wang X;Lu S;Lai D;Liu Y;Zhou L;Zhang G
稻曲病是水稻上一种日益严重的真菌病害。全球生产。稻曲菌素是一种双萘并-γ-吡喃酮类真菌毒素，以前从水稻稻曲病菌感染的稻穗中分离得到。为了研究水稻FSB中的主要黑曲霉素及其分布，从水稻FSB中分离得到5个主要的双萘并-γ-吡喃酮类化合物，即黑曲霉素A（1）、G（2）、B（3）、I（4）和C（5），并通过NMR、高分辨质谱和文献数据进行了结构鉴定。将早、中、晚熟水稻胚体分为不同部位，采用高效液相色谱法测定各部位5种主要的黑曲霉素类化合物的含量。结果表明，稻曲菌素类化合物的含量在水稻生育后期最高，生育中期次之。在发育初期，大多数类黑曲霉素分布于中层，占总量的96.4%。而在发育中后期，黑曲霉素主要分布于外、中层。在各成熟期水稻胚泡的内部都含有少量的黑曲霉素A（1）和G（2）。水稻FSBs中C-2和C-2'位不含羟甲基的类黑曲霉素A（1）和G（2）在生育前期含量较高，而C-2或C-2'位含羟甲基的类黑曲霉素B（3）、I（4）和C（5）在生育后期含量较高。
Rice false smut has become an increasingly serious fungal disease in rice (Oryza sativa L.) production worldwide. Ustilaginoidins are bis-naphtho-γ-pyrone mycotoxins previously isolated from the rice false smut balls (FSBs) infected by the pathogen Villosiclava virens in rice spikelets on panicles. To investigate the main ustilaginoidins and their distribution in rice FSBs, five main bis-naphtho-γ-pyrones, namely ustilaginoidins A (1), G (2), B (3), I (4) and C (5), were isolated and identified by NMR and high-resolution mass spectrometry as well as by comparison with the data in the literature. The rice FSBs at early, middle and late maturity stages were divided into their different parts and the contents of five main ustilaginoidins for each part were determined by HPLC analysis. The results revealed that the highest levels of ustilaginoidins were in late stage rice FSBs, followed by those at middle stage. Most ustilaginoidins, 96.4% of the total quantity, were distributed in the middle layer at early stage. However, ustilaginoidins were mainly distributed in the outer and middle layers at middle and late stages. Small amounts of ustilaginoidins A (1) and G (2) were found in the inner part of rice FSBs at each maturity stage. The contents of ustilaginoidins A (1) and G (2) without hydroxymethyl groups at C-2 and C-2’ of the γ-pyrone rings in rice FSBs were relatively high at early stage, while the contents of ustilaginoidins B (3), I (4), and C (5) with hydroxymethyl groups at C-2 or C-2’ were relatively high at late stage.