Metabolic Alterations in Two Cirsium Species Identified at Distinct Phenological Stages using UPLC-QTOF/MS
Metabolic Alterations in Two Cirsium Species Identified at Distinct Phenological Stages using UPLC-QTOF/MS
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DOI：
10.1002/pca.2716
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Hwang, Geum-Sook
中科院分区：
文献类型：
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作者：
Kim, Min-Sun;Nam, Miso;Hwang, Geum-Sook
简介Cirsium chanroenicum 和 C. setidens 常用于传统民间医学和作为食物来源。不同品种蓟在不同采收时期的品质与其代谢物组成有关，代谢物组成由物候阶段决定。目的利用超高效液相色谱（UPLC）四极杆飞行时间（QTOF）质谱（MS）测定两种蓟在不同物候阶段代谢物组成的差异。方法学在花期采集Cirsium chanroenicum和C. setidens植物。萌芽期和盛花期。使用 UPLC-QTOF/MS 测定蓟提取物的代谢谱，以表征物候阶段之间的差异，并使用 UPLC-QTOF/MS 多反应监测 (MRM) 定量主要代谢物。 结果在盛花期，酚酸以及苯丙素途径成分的水平增加。黄酮类化合物在两个物种的盛花期均占主导地位。两种蓟的香豆酸、山奈酚和果胶苷元的含量不同。总体而言，这些结果表明苯丙素代谢途径的组成部分在蓟的完整开花阶段上调，尽管我们确实观察到物种之间的一些差异。结论这些结果将有助于阐明与营养周期不同阶段相关的代谢途径，并可能有助于确定生物活性化合物含量最高的蓟收获的最佳季节。版权所有 (c) 2017 John Wiley & Sons, Ltd。为了表征花芽期和盛花期之间的差异，我们使用超高效液相色谱 (UPLC)-四极杆飞行时间 (QTOF) 质谱 (MS) 测定了蓟提取物的代谢特征，并使用 UPLC-QTOF/MS-多反应监测 (MRM) 对主要代谢物进行了定量。苯丙素途径中的酚酸和黄酮类化合物导致了两个物候阶段之间的差异。这是关于不同物候阶段蓟物种代谢变化的第一份报告，将有助于确定未来该植物的最佳收获时间。
IntroductionCirsium chanroenicum and C. setidens are commonly used both in traditional folk medicine and as a food source. The quality of different species of Cirsium at different harvest times is a function of their metabolite composition, which is determined by the phenological stage.ObjectiveWe sought to determine the differences in the metabolite composition of two species of Cirsium during different phenological stages using ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight (QTOF) mass spectrometry (MS).MethodologyCirsium chanroenicum and C. setidens plants were collected at the floral budding and full flowering stages. Metabolic profiles of Cirsium extracts were determined using UPLC-QTOF/MS to characterise the differences between phenological stages, and the major metabolites were quantified using UPLC-QTOF/MS-multiple reaction monitoring (MRM).ResultsAt the full flowering stage, the levels of phenolic acids as well as components of the phenylpropanoid pathway were increased. Flavonoids predominated at the full flowering stage in both species. The levels of coumaric acid, kaempferol, and pectolinarigenin differed between the two species of Cirsium. Overall, these results suggest that components of the phenylpropanoid metabolic pathway are upregulated in the full flowering stage in Cirsium, although we did observe some variation between the species.ConclusionThese results will help elucidate the metabolic pathways related to the different phases of the vegetative cycle, and may help determine the optimal season for the harvest of Cirsium with the highest levels of bioactive compounds. Copyright (c) 2017 John Wiley & Sons, Ltd.To characterise differences between the floral budding and full flowering stages, we determined the metabolic profile of Cirsium extracts using ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QTOF) mass spectrometry (MS), and the major metabolites were quantified using UPLC-QTOF/MS-multiple reaction monitoring (MRM). Phenolic acids and flavonoids of the phenylpropanoid pathway contributed to the differences between the two phenological stages. This is the first report of metabolite changes in Cirsium species at different phenological stages and will help determine the optimal harvest time for the plant in the future.