The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during Arabidopsis seed development
The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during Arabidopsis seed development
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DOI：
10.1111/tpj.13118
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发表时间：
2016-02-01
期刊：
影响因子：
7.2
通讯作者：
Bentsink, Leonie
中科院分区：
文献类型：
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作者：
Dekkers, Bas J. W.;He, Hanzi;Bentsink, Leonie
种子表达的DELAY OF GERMINATION（DOG）1基因是诱导休眠所必需的。除了非休眠表型，dog 1 -1突变体的特征还在于种子寿命缩短，这表明DOG 1也可能影响其他种子过程。然而，这方面几乎没有研究，也很难理解。为了揭示DOG 1在种子中的其他作用，我们使用转录组学和代谢组学对dog 1突变体进行了详细分析，以研究功能失调的DOG 1基因的分子后果。此外，我们使用遗传方法，利用弱阿坝不敏感（abi）3-1等位基因作为与dog 1 -1杂交的致敏遗传背景。DOG 1影响数百个基因的表达，包括晚期胚胎发生丰度和热休克蛋白基因，这些基因部分地通过控制ABI 5表达而受到DOG 1的影响。此外，在dog 1 -1突变体中，发现通常在种子成熟过程中积累的初级代谢产物的子集的含量受到影响。令人惊讶的是，abi 3 -1 dog 1 -1双突变体产生了对阿坝高度不敏感的绿色种子，表型模仿严重的abi 3突变体，表明dog 1 -1作为弱abi 3 -1等位基因的增强子，从而揭示了两个基因之间的遗传相互作用。对dog 1和dog 1 abi 3突变体的分析揭示了另外的种子表型，因此我们假设DOG 1的功能不限于休眠，而是种子成熟的多个方面所需的，部分是通过干扰阿坝信号传导组分。由于未知的机制，DOG 1是一种功能未知的蛋白质。在这里，我们表明，DOG 1在种子成熟中起着更普遍的作用，部分通过干扰几个阿坝信号成分。
The seed expressed gene DELAY OF GERMINATION (DOG) 1 is absolutely required for the induction of dormancy. Next to a non-dormant phenotype, the dog1-1 mutant is also characterized by a reduced seed longevity suggesting that DOG1 may affect additional seed processes as well. This aspect however, has been hardly studied and is poorly understood. To uncover additional roles of DOG1 in seeds we performed a detailed analysis of the dog1 mutant using both transcriptomics and metabolomics to investigate the molecular consequences of a dysfunctional DOG1 gene. Further, we used a genetic approach taking advantage of the weak aba insensitive (abi) 3-1 allele as a sensitized genetic background in a cross with dog1-1. DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. Furthermore, the content of a subset of primary metabolites, which normally accumulate during seed maturation, was found to be affected in the dog1-1 mutant. Surprisingly, the abi3-1 dog1-1 double mutant produced green seeds which are highly ABA insensitive, phenocopying severe abi3 mutants, indicating that dog1-1 acts as an enhancer of the weak abi3-1 allele and thus revealing a genetic interaction between both genes. Analysis of the dog1 and dog1 abi3 mutants revealed additional seed phenotypes and therefore we hypothesize that DOG1 function is not limited to dormancy but that it is required for multiple aspects of seed maturation, in part by interfering with ABA signalling components.Significance StatementDELAY OF GERMINATION1 (DOG1) is required for inducing seed dormancy but dog1 mutants also exhibit reduced seed longevity, due to an unknown mechanism, as DOG1 is a protein of unknown function. Here we show that DOG1 plays a more general role in seed maturation, in part by interfering with several ABA signalling components.