RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture
RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture
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大豆生命周期中的RNA沉默：与植物结构相关的多重限制系统和时空变异
DOI：
10.1007/s11248-017-0011-8
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Mori;A.;Sato;H.;Kasai;M.;Yamada;T. and Kanazawa;A.
导入植物基因组的转基因的表达有时被RNA沉默抑制。虽然已经研究了RNA沉默的局部和系统传播，但对转基因沉默在个体植物之间或不同世代植物之间的空间和时间变化的潜在机制知之甚少，这似乎是随机发生的。在这里，我们分析了单个大豆转化体后代多代中绿色荧光蛋白（GFP）基因RNA沉默的发生、传播和传播。在T3-T5代的整株植物中观察GFP荧光表明GFP沉默的起始和随后的传播在个体之间不同，尽管这种GFP沉默最常开始于初生叶。此外，GFP沉默可以扩散到种皮组织的外层，但在胚中几乎检测不到。这些结果与转基因沉默涉及其重置在生殖阶段，启动后发芽，并在每一代的系统性spreading. GFP沉默是不存在的枕，表明其皮层细胞抑制细胞间的传播或RNA沉默的诱导。GFP沉默的程度在茎和叶柄之间或小叶柄之间可能不同，小叶柄之间有有限的维管束连接它们，从而阻止沉默的长距离移动。两者合计，这些观察结果表明，启动和/或传播的RNA沉默取决于特定的功能，除了可以在高等植物中保守的机制的架构的植物。
The expression of transgenes introduced into a plant genome is sometimes suppressed by RNA silencing. Although local and systemic spread of RNA silencing have been studied, little is known about the mechanisms underlying spatial and temporal variation in transgene silencing between individual plants or between plants of different generations, which occurs seemingly stochastically. Here, we analyzed the occurrence, spread, and transmission of RNA silencing of the green fluorescent protein (GFP) gene over multiple generations of the progeny of a single soybean transformant. Observation of GFP fluorescence in entire plants of the T3–T5generations indicated that the initiation and subsequent spread ofGFPsilencing varied between individuals, although thisGFPsilencing most frequently began in the primary leaves. In addition,GFPsilencing could spread into the outer layer of seed coat tissues but was hardly detectable in the embryos. These results are consistent with the notion that transgene silencing involves its reset during reproductive phase, initiation after germination, and systemic spread in each generation.GFPsilencing was absent in the pulvinus, suggesting that its cortical cells inhibit cell-to-cell spread or induction of RNA silencing. The extent ofGFPsilencing could differ between the stem and a petiole or between petiolules, which have limited vascular bundles connecting them and thus deter long-distant movement of silencing. Taken together, these observations indicate that the initiation and/or spread of RNA silencing depend on specific features of the architecture of the plant in addition to the mechanisms that can be conserved in higher plants.