Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice.
Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice.
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DOI：
10.3390/nu7085297
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发表时间：
2015-08-06
期刊：
影响因子：
5.9
通讯作者：
Howard MT
中科院分区：
文献类型：
--
作者：
Tsuji PA;Carlson BA;Anderson CB;Seifried HE;Hatfield DL;Howard MT
硒是支持许多细胞功能和生化途径所需的必需元素。本研究的目的是研究降低膳食硒水平对基因表达的影响，以评估可能导致硒缺乏的生理后果的非硒蛋白基因表达的变化。给小鼠喂食缺乏硒或以亚硒酸钠形式补充硒的饮食六周。使用核糖体分析、RNA-Seq、微阵列和qPCR的组合测量肝脏mRNA表达和翻译的差异。应激相关硒蛋白mRNA的表达水平和翻译水平被证明是通过增加硒的状态上调，如参与炎症和对干扰素-γ的反应的基因。测量血清细胞因子水平的变化，这证实了干扰素-γ以及IL-6在硒充足的小鼠中增加。最后，肺组织的微阵列和qPCR分析表明，硒对免疫功能的影响不仅限于肝脏。这些数据与先前的报告一致，表明足够的硒水平可以支持有益的免疫应答，并进一步确定IL-6和干扰素-γ途径对膳食硒摄入量有反应。
Selenium is an essential element that is required to support a number of cellular functions and biochemical pathways. The objective of this study was to examine the effects of reduced dietary selenium levels on gene expression to assess changes in expression of non-selenoprotein genes that may contribute to the physiological consequences of selenium deficiency. Mice were fed diets that were either deficient in selenium or supplemented with selenium in the form of sodium selenite for six weeks. Differences in liver mRNA expression and translation were measured using a combination of ribosome profiling, RNA-Seq, microarrays, and qPCR. Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ. Changes in serum cytokine levels were measured which confirmed that interferon-γ, as well as IL-6, were increased in selenium adequate mice. Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver. These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake.