VASCULAR CELL-ADHESION MOLECULE-1 IS EXPRESSED IN HUMAN CORONARY ATHEROSCLEROTIC PLAQUES - IMPLICATIONS FOR THE MODE OF PROGRESSION OF ADVANCED CORONARY ATHEROSCLEROSIS
VASCULAR CELL-ADHESION MOLECULE-1 IS EXPRESSED IN HUMAN CORONARY ATHEROSCLEROTIC PLAQUES - IMPLICATIONS FOR THE MODE OF PROGRESSION OF ADVANCED CORONARY ATHEROSCLEROSIS
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DOI：
10.1172/jci116670
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发表时间：
1993-08-01
影响因子：
15.9
通讯作者：
ALPERS, CE
中科院分区：
文献类型：
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作者：
OBRIEN, KD;ALLEN, MD;ALPERS, CE
内皮附着是白细胞募集到动脉粥样硬化病变中的第一步。为了确定是否血管细胞粘附分子-1（VCAM-1）的表达可能发挥作用，在炎症细胞招募到人类动脉粥样硬化病变，免疫组化进行了多克隆兔抗血清，提出了对重组人VCAM-1，对24个动脉粥样硬化冠状动脉斑块和11个对照冠状动脉段与非动脉粥样硬化弥漫性内膜增厚10例。在相邻切片上进行免疫表型分析，以识别平滑肌细胞、巨噬细胞和内皮细胞。为了确认表达VCAM-1的细胞类型，用VCAM-1抗血清和每种细胞特异性标记物进行双重免疫染色，并进行原位杂交。所有动脉粥样硬化斑块都含有一些VCAM-1，而对照段为45%。在斑块（21%）和对照节段（27%）的动脉管腔处的内皮细胞上很少发现VCAM-1，但在斑块基底的新血管形成和炎症浸润区域中普遍存在。双重免疫染色和原位杂交证实，大多数VCAM-1表达的斑块平滑肌细胞和巨噬细胞的子集。结果证明了VCAM-1在人动脉粥样硬化中的存在，证明了体内人平滑肌细胞表达VCAM-1，并表明内膜新生血管可能是炎症细胞募集到晚期冠状动脉病变的重要部位。
Endothelial attachment is the initial step in leukocyte recruitment into developing atherosclerotic lesions. To determine whether vascular cell adhesion molecule-1 (VCAM-1) expression may play a role in inflammatory cell recruitment into human atherosclerotic lesions, immunohistochemistry was performed with a polyclonal rabbit antisera, raised against recombinant human VCAM-1, on 24 atherosclerotic coronary plaques and 11 control coronary segments with nonatherosclerotic diffuse intimal thickening from 10 patients. Immunophenotyping was performed on adjacent sections to identify smooth muscle cells, macrophages, and endothelial cells. To confirm VCAM-1-expressing cell types, double immunostaining with VCAM-1 antisera and each of the cell-specific markers and in situ hybridization were performed.All atherosclerotic plaques contained some VCAM-1, compared to 45% of control segments. VCAM-1 was found infrequently on endothelial cells at the arterial lumen in both plaques (21%) and in control segments (27%), but was prevalent in areas of neovascularization and inflammatory infiltrate in the base of plaques. Double immunostaining and in situ hybridization confirmed that most VCAM-1 was expressed by subsets of plaque smooth muscle cells and macrophages. The results document the presence of VCAM-1 in human atherosclerosis, demonstrate VCAM-1 expression by human smooth muscle cells in vivo, and suggest that intimal neovasculature may be an important site of inflammatory cell recruitment into advanced coronary lesions.