Observation of Colloidal Gold Particles on the Surface of Yeast Protoplasts wit UHR-LVSEM
Observation of Colloidal Gold Particles on the Surface of Yeast Protoplasts wit UHR-LVSEM
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UHR-LVSEM 观察酵母原生质体表面胶体金颗粒
DOI：
10.1093/oxfordjournals.jmicro.a050984
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
H. Yaguchi
中科院分区：
文献类型：
--
作者：
M. Osumi;N. Yamada;H. Kobori;H. Yaguchi
直到最近，混合物的背散射电子信号和二次电子（SE）信号的金标记的标本已被用于并行可视化的表面形貌和本地化的金颗粒在生物标本的扫描电子显微镜（SEM）。然而，使用一种新的超高分辨率场发射扫描电子显微镜（UHR-FESEM），S-900 LV，可以在低电压（1-3 kV）下仅使用SE模式同时观察表面结构和金颗粒
Until recently, a mixture of backscattered electron signal and secondary electron (SE) signal of gold-labeled specimens has been used for the concurrent visualization of the surface topography and localization of gold particles in biological specimens by scanning electron microscopy (SEM). Using a new ultra-high resolution field emission scanning electron microscope (UHR-FESEM), S-900 LV, however, it is possible to observe concurrently the surface structure and gold particles using only the SE mode at a low voltage (1-3 kV)