Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution
Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution
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失去藻类质子梯度调节 5 会增加活性氧清除和氢气释放
DOI：
10.1111/jipb.12502
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发表时间：
2016-12-01
影响因子：
11.4
通讯作者：
Huang, Fang
中科院分区：
文献类型：
--
作者：
Chen, Mei;Zhang, Jin;Huang, Fang
我们已经鉴定了hpm 91，一种缺乏质子梯度调节5（PGR 5）的衣原体突变体，其能够产生氢（H-2）25天，与野生型相比产量增加超过30倍。因此，hpm 91显示出比先前表征的pgr 5突变体更高的H-2产生能力。hpm 91的生理生化特性表明，延长H-2的生产是由于PSII的稳定性增强，这与增加活性氧（ROS）的清除能力在硫剥夺。这种抗ROS反应似乎保护光合电子传递链免受光氧化损伤，从而确保电子供应氢化酶。
We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H-2) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H-2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H-2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.