The anti-adherence activity and bactericidal effect of microparticulate silver additives in composite resin materials
The anti-adherence activity and bactericidal effect of microparticulate silver additives in composite resin materials
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DOI：
10.1016/j.archoralbio.2009.03.004
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Hahnel, Sebastian
中科院分区：
文献类型：
--
作者：
Buergers, Ralf;Eidt, Andreas;Hahnel, Sebastian
目的：树脂基复合材料易积聚微生物和菌斑，从而引起粘接剂周围继发龋。本体外研究的目的是评价负载有银微粒的树脂复合材料对变形链球菌的抗菌活性。设计：圆形标本（直径为10.0 mm）的树脂复合材料基质，该树脂复合材料基质负载有两种不同浓度的银添加剂3：0.3%; Comp0.6：0.6%）和一个未加载的参考复合基质（Comp 0：0%）。表面粗糙度R-a通过perthometer测量来评估，并且根据水接触角的疏水性通过计算机图像分析来确定。标本在S.通过使用生物荧光测定（阿利兰/刃天青）定量变形链球菌悬浮液（1小时，37 ℃）和粘附的链球菌。此外，通过活/死细胞标记结合荧光显微镜检查评估粘附细菌的活力。结果如下：观察到Comp 0（66.3度）、Comp0.3（76.7度）和Comp0.6（89.4度）的中值水接触角之间的统计学显著差异（p < 0.001）。三到四倍高的粘附量。在参比化合物0（12，093个相对荧光单位）上发现的变形杆菌的数量比在化合物0.3（4258 rfu）和化合物0.6（3292）上发现的要多（两者p < 0.001）。在Comp0.3（6.1%）和Comp0.6（10.1%）上发现了比Comp 0（0.5%）显著更高的死细胞百分比（两者均为p < 0.001）。结论：向树脂复合材料中添加微粒银增加了表面疏水性并减少了粘附链球菌的数量。同时，它增加了复合材料表面上死亡和非活性细胞的百分比。因此，银添加剂似乎显示出抗粘附活性以及杀菌作用。(C)2009爱思唯尔有限公司保留所有权利。
Objective: Resin composite materials tend to accumulate microorganisms and dental plaque, which in turn may induce secondary caries around adhesive restorations. The aim of the present in vitro study was to evaluate the antibacterial activity of a resin composite material loaded with silver microparticles against Streptococcus mutans.Design: Circular specimens (10.0 mm in diameter) of a resin composite matrix loaded with two different concentrations of a silver additive (Comp0.3: 0.3%; Comp0.6: 0.6%) and one unloaded reference composite matrix (Comp0: 0%) were made. Surface roughness R-a was assessed by perthometer measurements and hydrophobicity according to water contact angles was determined by computerized image analysis. The specimens were incubated in a S. mutans suspension (1 h, 37 degrees C) and adhering streptococci were quantified by using a biofluorescence assay (Alamar blue/Resazurin). Additionally, the viability of adhering bacteria was assessed by live/dead cell labelling in combination with fluorescence microscopy. Results: Statistically significant differences between the median water contact angles of Comp0 (66.3 degrees), Comp0.3 (76.7 degrees), and Comp0.6 (89.4 degrees) were observed (p < 0.001). A three- to fourfold higher amount of adhering S. mutans was found on reference Comp0 (12,093 relative fluorescence units) than on Comp0.3 (4258 rfu) and Comp0.6 (3292) (p < 0.001 for both). Significantly higher percentages of dead cells than on Comp0 (0.5%) were found on Comp0.3 (6.1%) and on Comp0.6 (10.1%) (p < 0.001 for both).Conclusions: The addition of microparticulate silver to a resin composite material increased the surface hydrophobicity and reduced the number of adhering streptococci. Simultaneously it increased the percentage of dead and inactive cells on the composite surface. Thus, silver additives seem to demonstrate anti-adherence activity as well as a bactericidal effect. (C) 2009 Elsevier Ltd. All rights reserved.