Intrathecal production of interleukin-12 and gamma interferon in patients with bacterial meningitis
Intrathecal production of interleukin-12 and gamma interferon in patients with bacterial meningitis
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DOI：
10.1128/iai.65.3.877-881.1997
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发表时间：
1997-03-01
影响因子：
3.1
通讯作者：
deGroot, R
中科院分区：
文献类型：
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作者：
Kornelisse, RF;Hack, CE;deGroot, R
为探讨白介素12(IL-12)和干扰素(IFN-γ)在细菌性脑膜炎中的作用，对35例细菌性脑膜炎患儿和10例正常儿童血清和脑脊液(CSF)中活性IL-12(P70)、非活性亚基p40和干扰素-γ进行了检测。脑脊液中可检测到的干扰素-γ水平与IL-12 p40(r=0.40，P=0.02)和肿瘤坏死因子-α(r=0.46，P=0.04)呈正相关，但与IL-6、IL-8或IL-10无相关性。与脑脊液中的肿瘤坏死因子-α、IL-12和IL-10水平相反，肺炎球菌脑膜炎患者的干扰素-γ水平显著高于流感嗜血杆菌和脑膜炎奈瑟菌所致的脑膜炎患儿，可能是因为前者的脑脊液中有较高的肿瘤坏死因子-α/IL-10比率。我们认为，在细菌性脑膜炎急性期，IL-12和TNF-α诱导的干扰素-γ的产生可能参与了脑脊液室对微生物的天然免疫。
To assess the role of interleukin-12 (IL-12) and gamma interferon (IFN-gamma) in children with bacterial meningitis, bioactive IL-12 (p70) and the inactive subunit p40 and IFN-gamma were measured in serum and cerebrospinal fluid (CSF) from 35 children with bacterial meningitis and 10 control subjects, The production of IFN-gamma is induced by IL-12 with tumor necrosis factor alpha (TNF-alpha) as a costimulator and inhibited by IL-10, CSF concentrations of IL-12 p40 as well as those of IFN-gamma were markedly elevated, whereas IL-12 p70 was hardly detectable, Detectable CSF levels of IFN-gamma correlated positively with IL-12 p40 (r = 0.40, P = 0.02) and TNF-alpha (r = 0.46, P = 0.04) but not with IL-6, IL-8, or IL-10, In contrast to CSF levels of TNF-alpha, IL-12, and IL-10, those of IFN-gamma were significantly higher in patients with pneumococcal meningitis than in children with meningitis caused by Haemophilus influenzae and Neisseria meningitidis, presumably because of a high CSF TNF-alpha/IL-10 ratio in the former, We suggest that IL-12- and TNF-alpha-induced IFN-gamma production may contribute to the natural immunity against microorganisms in the CSF compartment during the acute phase of bacterial meningitis.