Mirrored Bilateral Slow-Wave Cortical Activity within Local Circuits Revealed by Fast Bihemispheric Voltage-Sensitive Dye Imaging in Anesthetized and Awake Mice
Mirrored Bilateral Slow-Wave Cortical Activity within Local Circuits Revealed by Fast Bihemispheric Voltage-Sensitive Dye Imaging in Anesthetized and Awake Mice
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DOI：
10.1523/jneurosci.6437-09.2010
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发表时间：
2010-03-10
影响因子：
5.3
通讯作者：
Murphy, Timothy H.
中科院分区：
文献类型：
--
作者：
Mohajerani, Majid H.;McVea, David A.;Murphy, Timothy H.
神经元膜电位的自发慢波振荡在啮齿动物皮层中大约每秒发生一次，并且可以用于形成诱发的神经元反应的功效并在睡眠期间巩固记忆。然而，这些振荡是否反映了所有皮层区域通过传播波的夹带，或者它们是否表现出区域和时间的异质性，反映了局部皮层回路中的处理是未知的。使用电压敏感染料（VSD）成像在成年C57 BL/6 J小鼠跨中线大开颅手术准备，我们记录了这种去极化活动，同时在麻醉和安静清醒的动物在两个皮层半球。VSD信号的自发振荡在两半球之间高度同步，并且胼胝体I/LnJ小鼠表明同步依赖于胼胝体。在麻醉和清醒的小鼠（从麻醉中恢复）中，振荡不一定是活动状态的全局变化，而是由复杂的局部模式组成，其特征在于多个离散峰不均匀地分布在皮层上。尽管去极化活动的局部模式很复杂，并且在几十毫秒内发生变化，但在胼胝体完整的小鼠中，它们在两个半球中都忠实地反映出来，这可能是为了确保两个半球中相关回路的平行修改。我们的结论是，在全球范围内的自发活动的节奏是复杂的事件，反映了协调处理局部皮层电路。
Spontaneous slow-wave oscillations of neuronal membrane potential occur about once every second in the rodent cortex and may serve to shape the efficacy of evoked neuronal responses and consolidate memory during sleep. However, whether these oscillations reflect the entrainment of all cortical regions via propagating waves or whether they exhibit regional and temporal heterogeneity that reflects processing in local cortical circuits is unknown. Using voltage-sensitive dye (VSD) imaging within an adult C57BL/6J mouse cross-midline large craniotomy preparation, we recorded this depolarizing activity across most of both cortical hemispheres simultaneously in both anesthetized and quiet awake animals. Spontaneous oscillations in the VSD signal were highly synchronized between hemispheres, and acallosal I/LnJ mice indicated that synchrony depended on the corpus callosum. In both anesthetized and awake mice (recovered from anesthesia), the oscillations were not necessarily global changes in activity state but were made up of complex local patterns characterized by multiple discrete peaks that were unevenly distributed across cortex. Although the local patterns of depolarizing activity were complex and changed over tens of milliseconds, they were faithfully mirrored in both hemispheres in mice with an intact corpus callosum, to perhaps ensure parallel modification of related circuits in both hemispheres. We conclude that within global rhythms of spontaneous activity are complex events that reflect orchestrated processing within local cortical circuits.