Neurogenic cells in human amniotic fluid
Neurogenic cells in human amniotic fluid
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DOI：
10.1016/j.ajog.2003.12.014
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发表时间：
2004-07-01
影响因子：
9.8
通讯作者：
Hengstschläger, M
中科院分区：
文献类型：
--
作者：
Prusa, AR;Marton, E;Hengstschläger, M
目的：本研究的目的是确定人类羊水中是否含有具有分化为神经源性细胞潜力的细胞。羊水细胞（未培养或在标准或神经源性分化培养基中培养）分析形态学神经源性分化和分化簇133的表达。（神经干细胞标记物），巢蛋白（神经元祖细胞）、神经丝（神经元）、p75共同神经营养素受体、脑源性神经营养因子和神经营养素-3和环核苷酸磷酸二酯酶结果：在未培养的细胞中未检测到神经源性细胞的出现，在标准培养基中培养后为零星的，但在神经源性分化培养基中强烈增加，并且伴随着所分析的标记基因的表达的诱导。这项研究首次提供了证据，证明人类羊水中含有表达神经干细胞和祖细胞标记物的细胞，这些细胞具有分化为神经源性细胞的潜力。(C)2004年爱思唯尔公司All rights reserved.
Objective: The purpose of this study was to determine whether human amniotic fluid contains cells that harbor the potential to differentiate into neurogenic cells.Study design: Amniotic fluid cells (uncultivated or cultivated in standard or in neurogenic differentiation medium) were analyzed for morphologic neurogenic differentiation and for expression of cluster of differentiation 133 (marker for neuronal stem cells), nestin (neuronal progenitor cells), neurofilament (neurons), the p75 common neurothrophin receptor, the brain-derived neurotrophic factor and neurotrophin-3 and cyclic nucleotide phosphodiesterase (oligodendrocytes).Results: The appearance of neurogenic cells was not detected in uncultivated cells, was sporadic after cultivation in standard medium but strongly increased in neurogenic differentiation medium, and was accompanied by the induction of the expression of the analyzed marker genes.Conclusion: For the first time, this study provides evidence that human amniotic fluid contains cells that express markers for neuronal stem and progenitor cells, which harbor the potential to differentiate into neurogenic cells. (C) 2004 Elsevier Inc. All rights reserved.