Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells
Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells
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DOI：
10.1016/j.biomaterials.2010.01.134
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Hayashi, Shuji
中科院分区：
文献类型：
--
作者：
Yukawa, Hiroshi;Kagami, Yukimasa;Hayashi, Shuji
量子点（QDs）被用于研究荧光探针对生物分子和细胞成像的影响。脂肪组织来源的干细胞具有相对较低的供体部位发病率，但在收获时产生大量干细胞，使用八精氨酸肽（R8）细胞穿透肽（CPP）进行QDs转导。QDs的浓度比为R8 = 1 × 10(4)。用小于16 nM的QDs655转导的ASCs未见细胞毒性。此外，bbb80 %的细胞在1 h内可被标记，荧光强度至少维持2周。用R8转导QDs的ASCs可以分化为成脂细胞和成骨细胞，这表明这些细胞保持了干细胞的潜能。用R8标记QDs的ASCs进一步皮下移植到小鼠背部或通过尾静脉移植到小鼠体内。使用Maestro体内成像系统可以对标记的ASCs进行成像，对比度很好。这些数据表明，使用R8进行QD标记可用于ASCs成像。(C) 2010 Elsevier Ltd.版权所有。
Quantum dots (QDs) have been used to study the effects of fluorescent probes for biomolecules and cell imaging. Adipose tissue-derived stem cells, which carry a relatively lower donor site morbidity, while yielding a large number of stem cells at harvest, were transduced with QDs using the octa-arginine peptide (R8) cell-penetrating peptide (CPP). The concentration ratio of QDs:R8 of 1 x 10(4) was optimal for delivery into ASCs. No cytotoxicity was observed in ASCs transduced with less than 16 nM of QDs655. In addition, >80% of the cells could be labeled within 1 h and the fluorescent intensity was maintained at least for 2 weeks. The ASCs transduced with QDs using R8 could be differentiated into both adipogenic and osteogenic cells, thus suggesting that the cells maintained their stem cell potency. The ASCs labeled with QDs using R8 were further transplanted subcutaneously into the backs of mice or into mice through the tail vein. The labeled ASCs could be imaged with good contrast using the Maestro in vivo imaging system. These data suggested that QD labeling using R8 could be utilized for the imaging of ASCs. (C) 2010 Elsevier Ltd. All rights reserved.