CORTICOTROPHIN-RELEASING FACTOR IN HUMAN-PLACENTA - LOCALIZATION, CONCENTRATION AND RELEASE INVITRO
CORTICOTROPHIN-RELEASING FACTOR IN HUMAN-PLACENTA - LOCALIZATION, CONCENTRATION AND RELEASE INVITRO
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DOI：
10.1016/0143-4004(88)90050-1
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发表时间：
1988-07-01
期刊：
影响因子：
3.8
通讯作者：
WAHLSTROM, T
中科院分区：
文献类型：
--
作者：
SAIJONMAA, O;LAATIKAINEN, T;WAHLSTROM, T
用免疫组织化学方法检测早孕胎盘细胞滋养细胞、蜕膜和羊膜中促肾上腺皮质激素释放因子(CRF)的免疫反应。这种定位不同于合体滋养层细胞中的促肾上腺皮质激素(ACTH)和β-内啡肽。体外培养的早孕和足月胎盘组织均有CRF的释放。妊娠早期和晚期胎盘/蜕膜提取液中CRF的平均含量为0.99±-。0.5 ng/g和19.7+-。3.1 ng/g。自然阴道分娩后采集的足月胎盘提取液和脐静脉血浆中CRF的含量略高于临产前选择性剖宫产采集的CRF。
Corticotrophin-releasing factor (CRF) immunoreactivity was demonstrated by immunohistochemical staining in the cytotrophoblast of the early pregnancy placenta, in the decidua and in the amnion. This localization is different from that of adrenocorticotrophic hormone (ACTH) and .beta.-endorphin, which are present in the syncytiotrophoblast. The release of immunoreactive CRF was demonstrated from both early and term placental tissues in vitro. The mean amounts of CRF in the early and term pregnancy placental/decidual extracts were 0.99 .+-. 0.5 ng/g and 19.7 .+-. 3.1 ng/g, respectively. A slightly greater amount of CRF was found in extracts from term placentae and in cord venous plasma collected after spontaneous vaginal delivery than in those collected at elective caesarean section performed before the beginning of labour.