The role of metal ions in ischemia/reperfusion injury in skin flaps.
The role of metal ions in ischemia/reperfusion injury in skin flaps.
复制标题
金属离子在皮瓣缺血/再灌注损伤中的作用。
DOI：
10.1016/0022-4804(89)90221-7
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hoopes,JE
中科院分区：
文献类型：
--
作者：
Yoon,JO;Im,MJ;Manson,PN;Bulkley,GB;Hoopes,JE
本文研究了金属催化的Haber-Weiss反应产生的羟自由基对大鼠缺血后皮瓣的损伤作用。阻断腹股沟岛状皮瓣静脉回流7小时后再灌注。在再灌注时，用去铁胺、CaNa 2 EDTA、组氨酸、水杨酸盐或载体（生理盐水）输注皮瓣。在另一个实验组中，过氧化氢的作用通过在再灌注时输注过氧化氢酶来评估。单剂量去铁胺（40 mg/kg），CaNa 2 EDTA（50 mg/kg），或组氨酸（50 mg/ kg）的治疗，显着增加皮瓣存活率从24至63，75，和63%，分别。大剂量水杨酸盐（80 mg/kg）可提高皮瓣存活率（至63%）：较小剂量（40 mg/kg）无改善。大剂量的过氧化氢酶可提高存活率（达88%）。结果表明，金属离子的存在下所需的自由基诱导的组织损伤的表达。过氧化氢似乎对这种伤害的产生至关重要。
The role of hydroxyl radical generation by the metal-catalyzed Haber-Weiss reaction in producing injury to postischemic skin flaps in rats was evaluated. The venous drainage from groin island flaps was occluded for 7 hr and then reperfused. The flaps were infused with either deferoxamine, CaNa2EDTA, histidine, salicylate, or vehicle (saline) at the time of reperfusion. In another experimental group, the role of hydrogen peroxide was evaluated by the infusion of catalase at the time of reperfusion. Treatment with a single dose of deferoxamine (40 mg/kg), CaNa2EDTA (50 mg/kg), or histidine (50 mg/ kg), significantly increased the flap survival rate from 24 to 63, 75, and 63%, respectively. A large dose of salicylate (80 mg/kg) improved the flap survival rate (to 63%): a smaller dose (40 mg/kg) offered no improvement. A large dose of catalase ameliorated the survival rate (to 88%). The results suggest that the presence of metal ions is required for the expression of free radical-induced tissue damage. Hydrogen peroxide appears to be essential for the production of this injury.