Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24.
Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24.
复制标题
DOI：
10.1111/j.1365-313x.2010.04299.x
复制
发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Mingli Xu;Tieqiang Hu;Sarah M. McKim;Jhadeswar Murmu;G. Haughn;S. Hepworth
中科院分区：
文献类型：
--
作者：
Mingli Xu;Tieqiang Hu;Sarah M. McKim;Jhadeswar Murmu;G. Haughn;S. Hepworth
向开花的转变是植物中受到严格控制的发育决策。在拟南芥中，LFY（LEAFY）和AP1（APETALA1）是这种转变的关键调节因子，这些基因在花序分生组织产生的原基中的表达赋予花的命运。在此，我们研究了结构调节因子BOP1（BLADE - ON - PETIOLE1）和BOP2在促进花分生组织特性方面的作用。功能缺失的bop1 bop2突变体在花序和花的结构上显示出细微的缺陷，但与lfy或ap1组合时，在花分生组织命运和确定性方面的协同缺陷就显现出来。最显著的变化发生在bop1 bop2 ap1 - 1三突变体中，花朵转变为高度分支的类似花序的枝条。我们的数据表明，BOP1/2在花原基中与LFY不同，其功能是上调AP1，并且这三种活性共同作用以下调开花时间调节因子，包括在第2阶段花分生组织中的AGAMOUS - LIKE24。随后，BOP1/2与LFY和AP1平行促进A类花器官的模式形成。遗传和生化证据支持这样一种模型，即BOP1/2通过与TGA bZIP转录因子（包括PERIANTHIA）直接相互作用而被招募到AP1的启动子上。这些数据揭示了BOP1/2在花转变过程中对改造枝条结构的重要辅助作用。
The transition to flowering is a tightly controlled developmental decision in plants. In Arabidopsis, LEAFY (LFY) and APETALA1 (AP1) are key regulators of this transition and expression of these genes in primordia produced by the inflorescence meristem confers floral fate. Here, we examine the role of architectural regulators BLADE-ON-PETIOLE1 (BOP1) and BOP2 in promotion of floral meristem identity. Loss-of-function bop1 bop2 mutants show subtle defects in inflorescence and floral architecture but in combination with lfy or ap1, synergistic defects in floral meristem fate and determinacy are revealed. The most dramatic changes occur in bop1 bop2 ap1-1 triple mutants where flowers are converted into highly branched inflorescence-like shoots. Our data show that BOP1/2 function distinctly from LFY to upregulate AP1 in floral primordia and that all three activities converge to down-regulate flowering-time regulators including AGAMOUS-LIKE24 in stage 2 floral meristems. Subsequently, BOP1/2 promote A-class floral-organ patterning in parallel with LFY and AP1. Genetic and biochemical evidence support the model that BOP1/2 are recruited to the promoter of AP1 through direct interactions with TGA bZIP transcription factors, including PERIANTHIA. These data reveal an important supporting role for BOP1/2 in remodeling shoot architecture during the floral transition.