Evaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilms.
Evaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilms.
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含光敏剂的超疏水表面对牙周生物膜抗菌治疗的评价。
DOI：
10.1016/j.jphotobiol.2022.112458
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Lyons,AlanM
中科院分区：
文献类型：
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作者：
Tonon,CarolineCoradi;Ashraf,Shoaib;deSouzaRastelli,AlessandraNara;Ghosh,Goutam;Hasan,Tayyaba;Xu,QianFeng;Greer,Alexander;Lyons,AlanM
抗菌光动力疗法(APDT)是控制牙周病生物膜的一种很有前途的方法。然而，诸如牙齿的染色、光敏剂(PS)与革兰氏阳性菌和革兰氏阴性菌的优先相互作用以及缺氧性牙周袋中的氧气不足等问题阻碍了其在临床上的应用。为了克服这些挑战，一种新型的产生空气中单线态氧的超疏水(SH)薄膜已经被开发出来。SH-APDT方法将PS隔离在拓扑粗糙的固体SH膜上，在该膜上的通道允许空气扩散到PS表面，从而确保足够的氧气供应。在光照下，产生气相单线态氧(1O2)，并从SH表面扩散到底层生物膜。通过集落形成单位计数、XTT和共聚焦显微镜观察生物膜代谢情况，评价其杀灭效果与透射量(17.9~89.5nJ/cm2)和Chorin e6载量(96~1110nmol/cm2)的关系。研究发现，在多物种生物膜中，革兰氏阳性和革兰氏阴性细菌的活力下降与通量以及PS的负载量呈线性关系，当使用1110nmol/cm2的氯素e6时，PS的负载量高达71.6nmol/cm2。在此条件下观察到4.6log细菌减少(p<；n0.05)。这种新的SH-APDT方法有望成为一种有效的方法来消毒与牙周病相关的多种细菌生物膜，并将在未来的动物模型中进行评估。
Antimicrobial photodynamic therapy (aPDT) is a promising approach to control biofilms involved in periodontal diseases. However, certain challenges, such as staining of teeth, preferential interaction of photosensitizer (PS) with Gram-positive versus Gram-negative bacteria, and insufficient oxygen in hypoxic periodontal pockets have presented barriers to its use in the clinic. To overcome these challenges, a novel superhydrophobic (SH) film that generates airborne singlet oxygen has been developed. The SH-aPDT approach isolates the PS onto a topologically rough solid SH film on which channels allow air to diffuse to the PS surface, thus ensuring sufficient oxygen supply. Upon illumination, gas phase singlet oxygen (1O2) is produced and diffuses from the SH surface to the underlying biofilm. The killing efficacy was assessed as a function of transmitted fluence (17.9–89.5 J/cm2) and chorin e6 loading (96–1110 nmol/cm2) by counting of colony forming units, biofilm metabolism by XTT and confocal microscopy. The decrease in viability of both Gram-positive and Gram-negative bacteria in a multi-species biofilm was found to be linearly dependent on the fluence as well as the loading of the PS up to 71.6 J/cm2when 1110 nmols/cm2of chlorin e6 was used. A > 4.6 log bacterial reduction was observed under these conditions (p< 0.05). This novel SH-aPDT approach shows promise as an effective method to disinfect multi-species bacterial biofilms associated with periodontal disease and will be evaluated in animal models in future studies.