Explant culture of human and rabbit articular chondrocytes.
Explant culture of human and rabbit articular chondrocytes.
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人和兔关节软骨细胞的外植体培养。
DOI：
10.3109/03008207809152628
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发表时间：
1978
影响因子：
2.9
通讯作者：
L. Sokoloff
中科院分区：
文献类型：
--
作者：
C. Malemud;D. Norby;L. Sokoloff
Copious outgrowth of chondrocytes was obtained by explantation from each of three rabbit and one surgically-resected human articular cartilages pretreated briefly with trypsin. In lapine explants, ascorbate (40 micrograms/ml) increased DNA three-fold over control values and resulted in deposition of a chondroid matrix. It doubled radiosulfate incorporation by the outgrowths. Up to 56% of the sulfated glycosaminoglycan synthesized was located in the trypsin-digestible pericellular coat compared with about 15% in previous monolayer cultures. The collagens synthesized were characterized partially. In rabbit cell cultures, the alpha 1:alpha 2 ratio varied from 2.9 to 3.8. In human cultures, an unusual post-alpha 2 peak was observed. The findings suggest an uncoupling of the phenotypic expression of the major cartilaginous macromolecules in the cultures. There were no distinctive differences between chondrocytes derived from normal and fibrillated human cartilage of the same individual.
