The influence of poly(ethylene oxide) grafting via siloxane tethers on protein adsorption
The influence of poly(ethylene oxide) grafting via siloxane tethers on protein adsorption
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DOI：
10.1016/j.biomaterials.2009.01.051
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发表时间：
2009-05-01
期刊：
影响因子：
14
通讯作者：
Grunlan, Melissa A.
中科院分区：
文献类型：
--
作者：
Murthy, Ranjini;Shell, Courtney E.;Grunlan, Melissa A.
将具有不同长度的硅氧烷系链的两亲性聚氧乙烯-硅烷(a-c)(通式为alpha-(EtO)(3)si-(CH2)(2)-oligodimethylsiloxane(n)-block-poly(ethylene氧化物)(8)-OCH_3[n=0(A)，n-4(B)和n=13(C)]接枝到硅片上，并测量了对血浆蛋白的吸附阻力。用低聚二甲基硅氧烷系链法将PEO链段与可水解性三乙氧基硅烷[(ETO)(3)Si]接枝基团分开，是一种将PEO链接枝到表面的新方法。将a-c接枝的表面与含有丙基间隔的传统PEO-硅烷[(ETO)(3)Si-(CH2)(3)-聚环氧乙烷(8)-OCH3，PEO对照]接枝的表面进行了比较。随着硅氧烷链长的增加，接枝到氧化硅片上的PEO-硅烷的链密度降低，疏水性增强，从而导致表面亲水性降低。尽管表面亲水性降低，但对牛血清白蛋白(BSA)的吸附阻力按如下顺序增加：PEO CONTROL<；a<；b近似于c，对人纤维蛋白原(HF)按以下顺序增加：PEO CONTROL<；a<；b<；c。
Amphiphilic PEO-silanes (a-c) having siloxane tethers of varying lengths with the general formula alpha-(EtO)(3)si-(CH2)(2)-oligodimethylsiloxane(n)-block-poly(ethylene oxide)(8)-OCH3 [n = 0 (a), n - 4 (b), and n = 13 (c)] were grafted onto silicon wafers and resistance to adsorption of plasma proteins was measured. Distancing the PEO segment from the hydrolyzable triethoxysilane [(EtO)(3)Si] grafting group by a oligodimethylsiloxane tether represents a new method of grafting PEO chains to surfaces. Properties of surfaces grafted with a-c were compared to surfaces grafted with a traditional PEO-silane containing a propyl spacer [(EtO)(3)Si-(CH2)(3)-poly(ethylene oxide)(8)-OCH3, PEO control]. As the siloxane tether length increased, chain density of PEO-silanes grafted onto oxidized silicon wafers decreased and hydrophobicity of the PEO-silane increased which led to a decrease in surface hydrophilicity. Despite decreased surface hydrophilicity, resistance to the adsorption of bovine serum albumin (BSA) increased in the order: PEO control < a < b approximate to c and to human fibrinogen (HF) increased in the order: PEO control < a < b < c. Published by Elsevier Ltd.