BASIC CALCIUM-PHOSPHATE CRYSTALS STIMULATE CELL-PROLIFERATION AND COLLAGENASE MESSAGE ACCUMULATION IN CULTURED ADULT ARTICULAR CHONDROCYTES
BASIC CALCIUM-PHOSPHATE CRYSTALS STIMULATE CELL-PROLIFERATION AND COLLAGENASE MESSAGE ACCUMULATION IN CULTURED ADULT ARTICULAR CHONDROCYTES
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DOI：
10.1002/art.1780350314
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发表时间：
1992-03-01
影响因子：
--
通讯作者：
CHEUNG, HS
中科院分区：
文献类型：
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作者：
MITCHELL, PG;STRUVE, JA;CHEUNG, HS
Objective. 研究碱性磷酸钙晶体（BCP）对原代培养猪软骨细胞有丝分裂和胶原酶基因转录的影响。 蛋白激酶在BCP晶体刺激的DNA合成中的作用进行了研究，使用胸苷掺入，激酶抑制剂，和蛋白激酶C（PKC）测定。 采用北方印迹法检测胶原酶c-fos和c-jun的表达。 BCP晶体以PKC依赖的方式刺激软骨细胞增殖。 胶原酶信使水平的增加伴随着c-fos的增加，而不是c-jun的增加。 BCP晶体可能有助于在某些风湿性疾病中观察到的软骨细胞增殖和胶原酶分泌异常。
Objective. To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.Methods. The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message.Results. BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun.Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.