Characterization of three serotonin receptors from the small white butterfly, Pieris rapae
Characterization of three serotonin receptors from the small white butterfly, Pieris rapae
复制标题
小白蝴蝶菜青虫三种血清素受体的表征
DOI：
10.1016/j.ibmb.2017.06.011
复制
发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
Huang, Jia
中科院分区：
文献类型：
--
作者：
Qi, Yi-xiang;Jin, Miao;Huang, Jia
5-羟色胺（5-hydroxytryptamine，5-HT）在调节原口动物和后口动物的多种生理过程和行为中起着关键作用。这些功能通过5-羟色胺与其受体的结合来介导，5-羟色胺被认为是潜在的杀虫剂靶标。本文研究了白色（Pierisraelae）的3种5-HT受体（Piera 5-HT 1A、Piera 5-HT 1B、Piera 5-HT 7）的序列、药理学和组织分布。5-HT激活Piera 5-HT 1A或Piera 5-HT 1B可抑制cAMP的产生，并呈剂量依赖性。用5-HT刺激Piera 5-HT 7可显着增加cAMP水平。令人惊讶的是，除了5-甲氧基色胺，激动剂包括α-甲基血清素，8-羟基-DPAT和5-羧酰胺色胺激活这些受体差。这一结果与以前在烟草中的发现一致。所有三种受体都被甲硫氨酸阻断，但酮色林和育亨宾无效。选择性哺乳动物5-HT受体拮抗剂SB 216641和SB 269970分别对Piera 5-HT 1B和Piera 5-HT 7有较强的抑制作用。本研究结果表明，鳞翅目昆虫5-HT受体的药理学特性与其它昆虫和脊椎动物有很大的不同，可能有助于开发新的选择性农药。本研究为进一步研究昆虫5-HT受体的功能提供了重要信息。(C)2017由Elsevier Ltd.出版
Serotonin (5-hydroxytryptamine, 5-HT) plays a key role in modulating diverse physiological processes and behaviors in both protostomes and deuterostomes. These functions are mediated through the binding of serotonin to its receptors, which are recognized as potential insecticide targets. We investigated the sequence, pharmacology and tissue distribution of three 5-HT receptors (Piera5-HT1A, Piera5-HT1B, Piera5-HT7) from the small white butterfly Pieris rapae, an important pest of cultivated cabbages and other mustard family crops. Activation of Piera5-HT1A or Piera5-HT1B by 5-HT inhibited the production of cAMP in a dose-dependent manner. Stimulation of Piera5-HT7 with 5-HT increased cAMP level significantly. Surprisingly, with the exception of 5-methoxytryptamine, agonists including alpha-methylserotonin, 8-Hydroxy-DPAT and 5-carboxamidotryptamine activated these receptors poorly. The results are consistent with previous findings in Manduca sexta. All three receptors were blocked by methiothepin, but ketanserin and yohimbine were not effective. The selective mammalian 5-HT receptor antagonists SB 216641 and SB 269970 displayed potent inhibition effects on Piera5-HT1B and Piera5-HT7 respectively. The results we achieved here indicate that the pharmacological properties of Lepidoptera 5-HT receptors are quite different from those in other insects and vertebrates and may contribute to development of new selective pesticides. This study offers important information on three 5-HT receptors from P. rapae that will facilitate further analysis of the functions of 5-HT receptors in insects. (C) 2017 Published by Elsevier Ltd.