Mechanical function of muscle reinnervated by end-to-side neurorrhaphy
Mechanical function of muscle reinnervated by end-to-side neurorrhaphy
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DOI：
10.1097/00006534-199906000-00017
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
Kuzon, WM
中科院分区：
文献类型：
--
作者：
Kalliainen, LK;Cederna, PS;Kuzon, WM
端侧神经吻合是一种在端到端神经吻合不可行的情况下进行周围神经重建的外科技术。为了确定端侧神经吻合作为一种神经修复方法的有效性，作者检验了零假设：端侧神经吻合与端端神经吻合在骨骼肌去神经和再神经功能上没有差异。成年Lewis大鼠接受左腓神经横断端到端神经外膜修复（n = 9）或腓神经远端残端到胫神经侧端侧修复（n = 8）。经过b个月的恢复期后，测量每只动物指长伸肌的等距力（F-o），计算比力（sF(o)）。神经细胞粘附分子（NCAM）免疫组化染色鉴定失神经支配肌纤维群体。端侧组平均指长伸肌质量（195 +/- 32 g）显著大于端侧组（146 +/- 55 g） （P < 0.05）。端侧组的动物指长伸肌中失神经纤维的比例（9.4 +/- 3.2%）明显高于端侧组（3.8 +/- 1.0%）（p < 0.05）。尽管两组肌肉质量较低，去神经纤维比例较高，但F-o和sF(o)在两组中均无显著差异。这些数据支持零假设，即在适当的情况下，端到端神经吻合与端到端神经吻合在全肌力量恢复和比力产生方面没有差异。
End-to-side neurorrhaphy is a surgical technique for peripheral nerve reconstruction when end-to-end neurorrhaphy is not an option. To define the effectiveness of end-to-side neurorrhaphy as a method of nerve repair, the authors tested the null hypothesis: there is no difference in the mechanical function of skeletal muscle denervated and reinnervated by end-to-side versus end-to-end neurorrhaphy. Adult Lewis rats underwent either transection and end-to-end epineurial repair of the left peroneal nerve (n = 9) or end-to-side repair of the distal stump of the peroneal nerve to the side of the tibial nerve (n = 8). After a B-month recovery period, isometric force (F-o) was measured, and specific force (sF(o)) was calculated for the extensor digitorum longus muscle of each animal. Immunohistochemical staining for neural cell adhesion molecule (NCAM) was performed to identify populations of denervated muscle fibers. The mean extensor digitorum longus muscle mass in the end-to-end group (195 +/- 32 g) was significantly greater than that of the end-to-side group (146 +/- 55 g) (P < 0.05). A significantly greater percentage of denervated fibers was identified in the extensor digitorum longus muscles of animals in the end-to-side group (9.4 +/- 3.2 percent) than in those in the end-to-end group (3.8 +/- 1.0 percent) (p < 0.05). Despite a lower muscle mass and a higher percentage of denervated fibers, neither F-o nor sF(o) was significantly different in the two groups. These data support the null hypothesis that, under appropriate circumstances, there is no difference in the recovery of whole muscle force and specific force production in muscles reinnervated by end-to-side versus end-to-end neurorrhaphy.