Nascent RNA interaction keeps PRC2 activity poised and in check.
Nascent RNA interaction keeps PRC2 activity poised and in check.
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DOI：
10.1101/gad.247940.114
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发表时间：
2014-09-15
影响因子：
10.5
通讯作者：
Reinberg D
中科院分区：
文献类型：
--
作者：
Kaneko S;Son J;Bonasio R;Shen SS;Reinberg D
polycomb - repression complex 2 （PRC2）定位于抑制基因的启动子区域，产生H3K27me2/3，但也定位于胚胎干细胞中缺乏H3K27me2/3的活性基因的新生转录本。Kaneko等人表明，RNA相互作用抑制含有SET结构域的蛋白，如PRC2。crispr介导的截断prc2相互作用的新生RNA挽救了prc2介导的H3K27me2/3沉积。这项研究支持了一个模型，即PRC2只能标记那些被转录抑制物沉默的基因的抑制。polycomb - suppression complex 2 （PRC2）通过在Lys27位点（H3K27me2/3）催化组蛋白H3的甲基化，促进抑制转录的染色质结构域的维持和遗传。然而，通过其EZH2亚基，PRC2也可以结合胚胎干细胞中缺乏H3K27me2/3的活性基因的新生转录物。这里，生化分析表明，RNA相互作用在体外非特异性地抑制含有SET结构域的蛋白，如PRC2。然而，crispr介导的截断prc2相互作用的新生RNA挽救了prc2介导的H3K27me2/3沉积。与新生转录物的相互作用抑制了PRC2的活性，这支持了一种模型，即PRC2只能标记那些被转录抑制物沉默的基因。
Polycomb-repressive complex 2 (PRC2) localizes to the promoter regions of repressed genes giving rise to H3K27me2/3 but also localizes to nascent transcripts from active genes that are devoid of H3K27me2/3 in embryonic stem cells. Kaneko et al. show that RNA interaction inhibits SET domain-containing proteins, such as PRC2. CRISPR-mediated truncation of a PRC2-interacting nascent RNA rescued PRC2-mediated deposition of H3K27me2/3. This study supports a model in which PRC2 can only mark for repression those genes silenced by transcriptional repressors. Polycomb-repressive complex 2 (PRC2) facilitates the maintenance and inheritance of chromatin domains repressive to transcription through catalysis of methylation of histone H3 at Lys27 (H3K27me2/3). However, through its EZH2 subunit, PRC2 also binds to nascent transcripts from active genes that are devoid of H3K27me2/3 in embryonic stem cells. Here, biochemical analyses indicated that RNA interaction inhibits SET domain-containing proteins, such as PRC2, nonspecifically in vitro. However, CRISPR-mediated truncation of a PRC2-interacting nascent RNA rescued PRC2-mediated deposition of H3K27me2/3. That PRC2 activity is inhibited by interactions with nascent transcripts supports a model in which PRC2 can only mark for repression those genes silenced by transcriptional repressors.
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影响因子：
16.8
作者：
Kaneko, Syuzo;Son, Jinsook;Shen, Steven S.;Reinberg, Danny;Bonasio, Roberto
通讯作者：
Bonasio, Roberto
影响因子：
10.5
作者：
Son J;Shen SS;Margueron R;Reinberg D
通讯作者：
Reinberg D
影响因子：
16
作者：
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通讯作者：
Lee, Jeannie T.
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影响因子：
12.3
作者：
Friedersdorf MB;Keene JD
通讯作者：
Keene JD
影响因子：
64.5
作者：
Peng JC;Valouev A;Swigut T;Zhang J;Zhao Y;Sidow A;Wysocka J
通讯作者：
Wysocka J